PSAT1 Antibody (GT1353) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13548
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (95%), Rabbit (94%), Rat (95%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT1353
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human PSAT1. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (82%)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images for PSAT1 Antibody (GT1353) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: PSAT1 Antibody (GT1353) [NBP3-13548]
Immunocytochemistry/Immunofluorescence: PSAT1 Antibody (GT1353) [NBP3-13548] - PSAT1 antibody [GT1353] detects PSAT1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PSAT1 protein stained by PSAT1 antibody [GT1353] (NBP3-13548) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.Western Blot: PSAT1 Antibody (GT1353) [NBP3-13548]
Western Blot: PSAT1 Antibody (GT1353) [NBP3-13548] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] (NBP3-13548) diluted at 1:1000.Immunocytochemistry/ Immunofluorescence: PSAT1 Antibody (GT1353) [NBP3-13548] -
Immunocytochemistry/ Immunofluorescence: PSAT1 Antibody (GT1353) [NBP3-13548] - PSAT1 antibody [GT1353] detects PSAT1 protein at cytoplasm by immunofluorescent analysis.Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PSAT1 stained by PSAT1 antibody [GT1353] (NBP3-13548) diluted at 1:500.
Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: PSAT1 Antibody (GT1353) [NBP3-13548] -
Immunohistochemistry-Paraffin: PSAT1 Antibody (GT1353) [NBP3-13548] - PSAT1 antibody [GT1353] detects secreted PSAT1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.
PSAT1 stained by PSAT1 antibody [GT1353] (NBP3-13548) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: PSAT1 Antibody (GT1353) [NBP3-13548] -
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [GT1353] (NBP3-13548) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Applications for PSAT1 Antibody (GT1353) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PSAT1
Long Name
Phosphoserine Aminotransferase 1
Alternate Names
EPIP, PSAT, PSATD
Gene Symbol
PSAT1
Additional PSAT1 Products
Product Documents for PSAT1 Antibody (GT1353) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PSAT1 Antibody (GT1353) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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