PSAT1 Antibody

Western Blot: PSAT1 Antibody [NBP1-32920]

Western Blot: PSAT1 Antibody [NBP1-32920] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3].

Western Blot: PSAT1 Antibody [NBP1-32920] -

PSAT1 antibody [N1C3] detects PSAT1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PSAT1 antibody [N1C3] (NBP1-32920) diluted at 1:1000.

Western Blot: PSAT1 Antibody [NBP1-32920] -

Loss of PSAT1 inhibits the metastatic potential of HCC cells containing p5372P. a Transwell assay showing the migration and invasion of parental Hep3B cells or PSAT1 knockout Hep3B cells transfected with vector, p53-72P, and p53-72R plasmids. Scale bar, 100 μm. b Immunoblotting analysis of indicated proteins in parental or PSAT1 knockout Hep3B cells transfected with vector, p53-72P, and p53-72R plasmids. c Transwell assay showing the migration and invasion of parental or PSAT1 knockout PLC/PRF/5 cells. Scale bar, 50 μm. d Wound healing assay showing cell migration of parental or PSAT1 knockout PLC/PRF/5 cells after 24 h. Scale bar, 200 μm. e Parental or PSAT1 knockout PLC/PRF/5 cells were intrahepatically injected into BALB/c nude mice and the lung metastases were then calculated. Scale bar, 200 μm. f Cell viability of parental or PSAT1 knockout PLC/PRF/5 cells treated with glucose restriction for 4 days. g Survival fraction of parental or PSAT1 knockout PLC/PRF/5 cells treated with or without 2.5 mM 2-DG. h Statistic dead cells of PLC/PRF/5 cells measured by Trypan blue staining. Parental or PSAT1 knockout PLC/PRF/5 cells were treated with or without poly-HEMA for 6 h. i Immunoblotting analysis of PSAT1 and beta -actin in PLC/PRF/5 and Huh7 cells treated with or without glucose restriction for 24 h. j Immunoblotting analysis of PSAT1 and beta -actin in indicated cells treated with or without poly-HEMA for 6 h. Data are means +/- s.d. NS not significant, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36788227), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: PSAT1 Antibody [NBP1-32920] -

PSAT1 is up-regulated in human metastatic HCC tissues and predicts poor prognosis. a, b Kaplan-Meier analysis of overall survival rate based on PSAT1 expression in HCC samples and TCGA-Liver dataset. c PSAT1 mRNA levels in different grades of HCC patients according to Oncomine data set Ye Liver. d PSAT1 mRNA levels in HCC patients with or without vascular invasion according to Oncomine dataset Wurmbach Liver. e PSAT1 mRNA levels in primary or metastatic tumor tissues according to Oncomine dataset Ye Liver. f PSAT1 mRNA levels in HCC tissues with or without metastases according to Oncomine dataset Ye Liver. g IHC analysis of PSAT1 protein level in HCC tissue samples with (n = 26) or without (n = 46) intrahepatic metastasis. Scale bar, 20 μm. Data are means +/- s.d. ***P < 0.001. h HE and IHC analysis of PSAT1 protein level in mice liver and lung (intrahepatical mice model). Scale bar, 1000 μm. Data are means +/- s.d. ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36788227), licensed under a CC-BY license. Not internally tested by Novus Biologicals.