PSMC3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86962

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PSMC3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EFPMPNEEARARIMQIHSRKMNVSPDVNYEELARCTDDFNGAQCKAVCVEAGMIALRRGATELTHEDYMEGILEVQAKKKANLQYY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSMC3 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PSMC3 Antibody [NBP1-86962]

Immunocytochemistry/ Immunofluorescence: PSMC3 Antibody [NBP1-86962]

Immunocytochemistry/Immunofluorescence: PSMC3 Antibody [NBP1-86962] - Staining of human cell line U-251 MG shows localization to nuclear bodies & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PSMC3 Antibody [NBP1-86962]

Immunohistochemistry-Paraffin: PSMC3 Antibody [NBP1-86962]

Immunohistochemistry-Paraffin: PSMC3 Antibody [NBP1-86962] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.
Simple Western: PSMC3 Antibody [NBP1-86962]

Simple Western: PSMC3 Antibody [NBP1-86962]

Simple Western: PSMC3 Antibody [NBP1-86962] - Mouse whole-spleen lysates at the indicated concentrations were probed with a 1:25 dilution of this PSMC3 antibody. Simple Western image submitted by a verified customer review.
Western Blot: PSMC3 Antibody [NBP1-86962]

Western Blot: PSMC3 Antibody [NBP1-86962]

Western Blot: PSMC3 Antibody [NBP1-86962] - Analysis of human fibroblasts. WB image submitted by a verified customer review.
PSMC3 Antibody - BSA Free Western Blot: PSMC3 Antibody - BSA Free [NBP1-86962]

Western Blot: PSMC3 Antibody - BSA Free [NBP1-86962]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
PSMC3 Antibody - BSA Free Western Blot: PSMC3 Antibody - BSA Free [NBP1-86962]

Western Blot: PSMC3 Antibody - BSA Free [NBP1-86962]

Analysis in human cell line MCF-7.

Applications for PSMC3 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in Mouse whole-spleen lysates, separated by Size, antibody dilution of 1:25

Reviewed Applications

Read 2 reviews rated 4 using NBP1-86962 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMC3

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq]

Alternate Names

human immunodeficiency virus tat transactivator binding protein-1, MGC8487, proteasome (prosome, macropain) 26S subunit, ATPase, 3,26S proteasome AAA-ATPase subunit RPT5, Proteasome 26S subunit ATPase 3, Proteasome subunit P50, TBP-126S protease regulatory subunit 6A, TBP1Tat-binding protein 1

Gene Symbol

PSMC3

Additional PSMC3 Products

Product Documents for PSMC3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PSMC3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PSMC3 Antibody - BSA Free

Customer Reviews for PSMC3 Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
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1 Stars
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Customer Images

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  • PSMC3 Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Mouse Spleen Lysate
    Species: Mouse
    Verified Customer | Posted 08/23/2019
    Whole-spleen lysates at the indicated concentrations were probed with a 1:25 dilution of this antibody.
    PSMC3 Antibody - BSA Free NBP1-86962
  • PSMC3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 04/19/2018
    PSMC3 Antibody - BSA Free NBP1-86962

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Protocols

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