PSMD11 Antibody (2C7) - BSA Free
Novus Biologicals | Catalog # NBP2-59484
Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2C7
Format
BSA Free
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Product Specifications
Immunogen
Recombinant human PSMD11 (1-422aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for PSMD11 Antibody (2C7) - BSA Free
Western Blot: PSMD11 Antibody (2C7)BSA Free [NBP2-59484]
Western Blot: PSMD11 Antibody (2C7) [NBP2-59484] - Lane 1: HeLa cell lysates, Lane 2: 293T cell lysates, Lane 3: U87-MG cell lysates, Lane 4: NIH-3T3 cell lysates, Lane 5: PC3 cell lysates, Lane 6: TF1 cell lysates, Lane 7: MCF7 cell lysatesImmunocytochemistry/ Immunofluorescence: PSMD11 Antibody (2C7) - BSA Free [NBP2-59484]
Immunocytochemistry/Immunofluorescence: PSMD11 Antibody (2C7) [NBP2-59484] - Analysis of PSMD11 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PSMD11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Western Blot: PSMD11 Antibody (2C7) - BSA Free [NBP2-59484] -
Simulated microgravity (sug) enhances PSMD11 expression in hPSCs.A Representative immunofluorescent micrographs of hPSCs cultured in sμg and 1g conditions and stained for PSMD11 and SOX2. DAPI was used to stain the nuclei of all cells. Scale bars, 200 um. Graphs showing the mean fluorescence intensity analysis from micrographs. B Representative Immunoblots and densitometry analysis of PSMD11 from protein lysates of cells cultured at sμg and 1g conditions. GAPDH was used as a loading control. Graphs on the right show the densitometry analysis of the immunoblots. C RT-qPCR analysis indicating the relative mRNA levels of PSMD11 present in cells cultured at sμg compared to 1g condition. *p < 0.05 (n = 3; unpaired t test). Error bars in graphs represent the SEM of the group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35787634), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for PSMD11 Antibody (2C7) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PSMD11
Alternate Names
MGC3844,26S proteasome regulatory subunit RPN6, p44.5,26S proteasome regulatory subunit 9, proteasome (prosome, macropain) 26S subunit, non-ATPase, 11,26S proteasome regulatory subunit p44.5, Rpn6,26S proteasome regulatory subunit S9, S9,26S proteasome non-ATPase regulatory subunit 11
Gene Symbol
PSMD11
Additional PSMD11 Products
Product Documents for PSMD11 Antibody (2C7) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PSMD11 Antibody (2C7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PSMD11 Antibody (2C7) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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