PSMD12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87801

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PSMD12 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: WQQALKSVVLYVILAPFDNEQSDLVHRISGDKKLEEIPKYKDLLKLFTTMELMRWSTLVEDYGMELRKGSLESPATDVFGSTEEGEKRWKDLKNRVVEH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSMD12 Antibody - BSA Free

Western Blot: PSMD12 Antibody [NBP1-87801]

Western Blot: PSMD12 Antibody [NBP1-87801]

Western Blot: PSMD12 Antibody [NBP1-87801] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: PSMD12 Antibody [NBP1-87801]

Immunocytochemistry/ Immunofluorescence: PSMD12 Antibody [NBP1-87801]

Immunocytochemistry/Immunofluorescence: PSMD12 Antibody [NBP1-87801] - Immunofluorescent staining of human cell line U-251 MG shows localization to microtubules.
Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801]

Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801]

Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801]

Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801]

Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801] - Staining of human liver shows distinct cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801]

Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801]

Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801] - Staining of human salivary gland shows low expression as expected.
Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801]

Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801]

Immunohistochemistry-Paraffin: PSMD12 Antibody [NBP1-87801] - Staining in human testis and salivary gland tissues using anti-PSMD12 antibody. Corresponding PSMD12 RNA-seq data are presented for the same tissues.

Applications for PSMD12 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMD12

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. [provided by RefSeq]

Alternate Names

26S proteasome non-ATPase regulatory subunit 12, 26S proteasome regulatory subunit p55, 26S proteasome regulatory subunit RPN5, MGC75406, p55, proteasome (prosome, macropain) 26S subunit, non-ATPase, 12, Rpn5

Gene Symbol

PSMD12

Additional PSMD12 Products

Product Documents for PSMD12 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PSMD12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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