PSMD8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83254

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PSMD8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NRVAEFHTELERLPAKDIQTNVYIKHPVSLEQYLMEGSYNKVFLAKGNIPAESYTFFIDILLDTIRDEIAGCIEKAYEKILFTEATRILFFNTPKKMTDYAKKRGWVLGPNNYYSFASQQQKPEDTTIPSTELAKQVIEYARQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSMD8 Antibody - BSA Free

Western Blot: PSMD8 Antibody [NBP1-83254]

Western Blot: PSMD8 Antibody [NBP1-83254]

Western Blot: PSMD8 Antibody [NBP1-83254] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: PSMD8 Antibody [NBP1-83254]

Immunocytochemistry/ Immunofluorescence: PSMD8 Antibody [NBP1-83254]

Immunocytochemistry/Immunofluorescence: PSMD8 Antibody [NBP1-83254] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254]

Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254]

Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254]

Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254]

Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254] - Staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254]

Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254]

Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254] - Staining of human pancreas shows very weak positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254]

Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254]

Immunohistochemistry-Paraffin: PSMD8 Antibody [NBP1-83254] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Applications for PSMD8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMD8

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 1. [provided by RefSeq]

Alternate Names

26S proteasome non-ATPase regulatory subunit 8, 26S proteasome regulatory subunit p31, HIP6,26S proteasome regulatory subunit S14, HYPF, MGC1660, Nin1p, p3126S proteasome regulatory subunit RPN12, proteasome (prosome, macropain) 26S subunit, non-ATPase, 8, Rpn12, S14

Gene Symbol

PSMD8

Additional PSMD8 Products

Product Documents for PSMD8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PSMD8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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