PSMF1 Antibody - Isoform 1

Novus Biologicals | Catalog # NB100-1178

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Peptide ELISA, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry Control

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PSMF1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DHLPPPGYDDMYL corresponding to C-Terminus according to NP_006805.2.

Specificity

This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PSMF1 Antibody - Isoform 1

Western Blot: PSMF1 AntibodyIsoform 1 [NB100-1178]

Western Blot: PSMF1 AntibodyIsoform 1 [NB100-1178]

Western Blot: PSMF1 Antibody - Isoform 1 [NB100-1178] - Staining of KNRK (A), NIH3T3 (B) with antibody at 1 ug/mL and Neuro2a (C) cell lysate with 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PSMF1 Antibody - Isoform 1 [NB100-1178]

Immunocytochemistry/ Immunofluorescence: PSMF1 Antibody - Isoform 1 [NB100-1178]

Immunocytochemistry/Immunofluorescence: PSMF1 Antibody - Isoform 1 [NB100-1178] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Western Blot: PSMF1 AntibodyIsoform 1 [NB100-1178]

Western Blot: PSMF1 AntibodyIsoform 1 [NB100-1178]

Western Blot: PSMF1 Antibody - Isoform 1 [NB100-1178] - Staining of HEK293 (A), K562 (B) with antibody at 0.3 ug/mL and LNCaP (C) cell lysate with 1 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for PSMF1 Antibody - Isoform 1

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL
Application Notes
Use in Flow-intracellular reported in scientific literature (PMID: 19015396).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMF1

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene.

Alternate Names

hPI31, PI31, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), proteasome inhibitor hP131 subunit, proteasome inhibitor PI31 subunit

Entrez Gene IDs

9491 (Human); 228769 (Mouse)

Gene Symbol

PSMF1

UniProt

NP_006805 (Human)

Additional PSMF1 Products

Product Documents for PSMF1 Antibody - Isoform 1

Certificate of Analysis

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Product Specific Notices for PSMF1 Antibody - Isoform 1

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PSMF1 Antibody - Isoform 1

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Protocols

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