PSRC2 Antibody

Novus Biologicals | Catalog # NB100-68267

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Knockout Validated, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PSRC2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1939 and 1989 of human coiled-coil domain containing 131 using the numbering given in entry NP_659419.3 (GeneID 196441).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PSRC2 Antibody

Western Blot: PSRC2 Antibody [NB100-68267]

Western Blot: PSRC2 Antibody [NB100-68267]

Western Blot: PSRC2 Antibody [NB100-68267] - Detection of Human CCDC131 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCDC131 antibody NB100-68267 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
PSRC2 Antibody [NB100-68267] - Detection of human CCDC131/PSRC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CCDC131 antibody NB100-68267 (lot 2 ) used for IP at 6 ug per reaction. CCDC131 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-CCDC131 antibody NB100-68265. For blotting immunoprecipitated CCDC131, NB100-68267 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for PSRC2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Knockout validation (PMID: 31722198). Use in ICC/IF reported in scientific literature (PMID:31950173).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PSRC2

CCDC131 (coiled-coil domain containing protein 131) is an uncharacterized proline/serine rich coiled-coil protein that bears 6 HAT repeats- and 5 TPR repeats. Coiled-coil proteins exhibit tertiary structures made up of a number of highly organized alpha helices wound around each other to mediate oligomerization. HAT (half a TPR) repeats form amphipathic alpha-helices and are similar to TPR repeats which are 34 amino acid repeat motifs which form alpha helical structures.

Alternate Names

coiled-coil domain containing 131, Coiled-coil domain-containing protein 131, DKFZp686A0722, KIAA0546CCDC131, MGC23401, MGC90200, proline/serine-rich coiled-coil 2, Proline/serine-rich coiled-coil protein 2, PSRC2, zinc finger C3H1 domain-containing protein, zinc finger, C3H1-type containing

Entrez Gene IDs

196441 (Human)

Gene Symbol

ZFC3H1

UniProt

NP_659419.3 (Human)

Additional PSRC2 Products

Product Documents for PSRC2 Antibody

Certificate of Analysis

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Product Specific Notices for PSRC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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