PTEN Antibody (CL11723) - BSA Free

Novus Biologicals | Catalog # NBP3-21201

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL11723

Format

BSA Free
View all PTEN Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P60484, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for PTEN Antibody (CL11723) - BSA Free

Western Blot: PTEN Antibody [NBP3-21201] -

Western Blot: PTEN Antibody [NBP3-21201] -

Analysis in human cell line CAPAN-2.
Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Staining of human cerebellum shows strong nuclear positivity in cells in molecular layer.
Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Staining of human endometrium shows strong nuclear positivity in stromal cells.
Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Analysis in human endometrium and skeletal muscle tissues using NBP3-21201 antibody. Corresponding PTEN RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Immunohistochemistry-Paraffin: PTEN Antibody [NBP3-21201] -

Staining of human glioblastoma multiforme shows strong positivity in tumor cells.

Applications for PTEN Antibody (CL11723) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTEN

PTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k/AKT signaling pathway (1-3). PTEN structural domains includes an N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PD biding sequence (5). Phosporylation of the tail of the protein will suppress its activity and modify its conformation to a

Long Name

Phosphatase and Tensin Homolog Deleted on Chromosome 10

Alternate Names

MMAC1

Gene Symbol

PTEN

Additional PTEN Products

Product Documents for PTEN Antibody (CL11723) - BSA Free

Certificate of Analysis

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Product Specific Notices for PTEN Antibody (CL11723) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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