PTEN [p Ser380] Antibody (JJ08-81)

Name: PTEN [p Ser380] Antibody (JJ08-81)
Brand: Novus Biologicals
SKU: NBP3-32791
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-32791

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JJ08-81 expressed in HEK293

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Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser380 of human PTEN. (Uniprot: P60484)

Modification

p Ser 380

Localization

Cytoplasm, Nucleus, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTEN [p Ser380] Antibody (JJ08-81)

Immunocytochemistry/ Immunofluorescence: PTEN [p Ser380] Antibody (JJ08-81) [NBP3-32791]

Immunocytochemistry/ Immunofluorescence: PTEN [p Ser380] Antibody (JJ08-81) [NBP3-32791] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32791, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Applications for PTEN [p Ser380] Antibody (JJ08-81)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTEN

PTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k/AKT signaling pathway (1-3). PTEN structural domains includes an N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PD biding sequence (5). Phosporylation of the tail of the protein will suppress its activity and modify its conformation to a

Long Name

Phosphatase and Tensin Homolog Deleted on Chromosome 10

Alternate Names

MMAC1

Gene Symbol

PTEN

Additional PTEN Products

Product Documents for PTEN [p Ser380] Antibody (JJ08-81)

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Certificate of Analysis

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Product Specific Notices for PTEN [p Ser380] Antibody (JJ08-81)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.