PTGFR Antibody - BSA Free

Novus Biologicals | Catalog # NLS1049

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine, Equine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PTGFR Primary Antibodies »

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from 2nd extracellular domain of human PTGFR.

Epitope

2nd extracellular domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse (88%), Rat (88%), Canine (88%), Sheep (81%), Bovine (81%), Hamster (81%), Rabbit (81%).

Specificity

Human PTGFR. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for PTGFR Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PTGFR Antibody - BSA Free [NLS1049]

Immunocytochemistry/ Immunofluorescence: PTGFR Antibody - BSA Free [NLS1049]

PTGFR-Antibody-Immunocytochemistry-Immunofluorescence-NLS1049-img0007.jpg
Immunohistochemistry-Paraffin: PTGFR Antibody - BSA Free [NLS1049]

Immunohistochemistry-Paraffin: PTGFR Antibody - BSA Free [NLS1049]

Immunohistochemistry-Paraffin: PTGFR Antibody [NLS1049] - Analysis of anti-FP / PTGFR antibody with human skin, melanoma.
Immunohistochemistry-Paraffin: PTGFR Antibody - BSA Free [NLS1049]

Immunohistochemistry-Paraffin: PTGFR Antibody - BSA Free [NLS1049]

Immunohistochemistry-Paraffin: PTGFR Antibody [NLS1049] - Analysis of anti-FP / PTGFR antibody with transfection control.
Immunohistochemistry-Paraffin: PTGFR Antibody - BSA Free [NLS1049]

Immunohistochemistry-Paraffin: PTGFR Antibody - BSA Free [NLS1049]

Immunohistochemistry-Paraffin: PTGFR Antibody [NLS1049] - Analysis of anti-FP / PTGFR antibody with human ovary.
Immunohistochemistry-Paraffin: PTGFR Antibody - BSA Free [NLS1049]

Immunohistochemistry-Paraffin: PTGFR Antibody - BSA Free [NLS1049]

Immunohistochemistry-Paraffin: PTGFR Antibody [NLS1049] - Analysis of anti-FP / PTGFR antibody with transfected cells expressing Prostaglandin F2 alpha Receptor at 4 ug/ ml.
PTGFR Antibody [NLS1049] - Anti-PTGFR antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
PTGFR Antibody - BSA Free

Western Blot: PTGFR Antibody - BSA Free [NLS1049] -

Presence of FPr in porcine corpora lutea (pCLs) at the early-luteal phase (EL-p), the mid-luteal phase (ML-p) and during pregnancy (P-p). A) Relative FPr mRNA expression. The results are presented as Delta Ct (HPRT Ct - FPr Ct). B) FPr protein content. The results are presented as AU (arbitrary units). Data represent mean ± SD. Different letters indicate statistically significant differences (P < 0.05). C) Representative Western blotting of FPr.
PTGFR Antibody - BSA Free

Western Blot: PTGFR Antibody - BSA Free [NLS1049] -

Presence of FPr in porcine corpora lutea-derived microvascular endothelial cells (pCL-MVECs) and porcine Aortic Endothelial Cells (pAECs). A) Relative FPr mRNA expression. The results are presented as Delta Ct (HPRT Ct - FPr Ct). B) FPr protein content. The results are presented as AU (arbitrary units). Data represent mean ± SD. Different letters indicate statistically significant differences (P < 0.05). C) Representative Western blotting of FPr.
PTGFR Antibody - BSA Free

Western Blot: PTGFR Antibody - BSA Free [NLS1049] -

Representative FPr immunofluorescent staining on porcine corpus luteum-microvascular endothelial cells (pCL-MVECs) isolated from the early luteal phase (EL-p) (A), the mid-luteal phase (ML-p) (B) and during pregnancy (P-p) (C) (×400). D) porcine Aortic Endothelial Cell (pAEC) culture (x400). Representative immunostaining of the FPr on non-fixed EL-p pCL-MVECs is shown (E).
PTGFR Antibody - BSA Free

Western Blot: PTGFR Antibody - BSA Free [NLS1049] -

Western Blot: PTGFR Antibody - BSA Free [NLS1049] - Presence of FPr in porcine corpora lutea (pCLs) at the early-luteal phase (EL-p), the mid-luteal phase (ML-p) & during pregnancy (P-p). A) Relative FPr mRNA expression. The results are presented as Delta Ct (HPRT Ct - FPr Ct). B) FPr protein content. The results are presented as AU (arbitrary units). Data represent mean ± SD. Different letters indicate statistically significant differences (P < 0.05). C) Representative Western blotting of FPr. Image collected & cropped by CiteAb from the following publication (https://rbej.biomedcentral.com/articles/10.1186/1477-7827-5-31), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PTGFR Antibody - BSA Free

Western Blot: PTGFR Antibody - BSA Free [NLS1049] -

Western Blot: PTGFR Antibody - BSA Free [NLS1049] - Representative FPr immunofluorescent staining on porcine corpus luteum-microvascular endothelial cells (pCL-MVECs) isolated from the early luteal phase (EL-p) (A), the mid-luteal phase (ML-p) (B) & during pregnancy (P-p) (C) (×400). D) porcine Aortic Endothelial Cell (pAEC) culture (x400). Representative immunostaining of the FPr on non-fixed EL-p pCL-MVECs is shown (E). Image collected & cropped by CiteAb from the following publication (https://rbej.biomedcentral.com/articles/10.1186/1477-7827-5-31), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PTGFR Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PTGFR Antibody - BSA Free [NLS1049] -

Immunocytochemistry/ Immunofluorescence: PTGFR Antibody - BSA Free [NLS1049] - Representative FPr immunofluorescent staining on porcine corpus luteum-microvascular endothelial cells (pCL-MVECs) isolated from the early luteal phase (EL-p) (A), the mid-luteal phase (ML-p) (B) & during pregnancy (P-p) (C) (×400). D) porcine Aortic Endothelial Cell (pAEC) culture (x400). Representative immunostaining of the FPr on non-fixed EL-p pCL-MVECs is shown (E). Image collected & cropped by CiteAb from the following publication (https://rbej.biomedcentral.com/articles/10.1186/1477-7827-5-31), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PTGFR Antibody - BSA Free

Western Blot: PTGFR Antibody - BSA Free [NLS1049] -

Western Blot: PTGFR Antibody - BSA Free [NLS1049] - Presence of FPr in porcine corpora lutea-derived microvascular endothelial cells (pCL-MVECs) & porcine Aortic Endothelial Cells (pAECs). A) Relative FPr mRNA expression. The results are presented as Delta Ct (HPRT Ct - FPr Ct). B) FPr protein content. The results are presented as AU (arbitrary units). Data represent mean ± SD. Different letters indicate statistically significant differences (P < 0.05). C) Representative Western blotting of FPr. Image collected & cropped by CiteAb from the following publication (https://rbej.biomedcentral.com/articles/10.1186/1477-7827-5-31), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PTGFR Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

4-8 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: PTGFR

The prostaglandin F2-alpha receptor is a member of the Prostanoid Receptor subfamily. Along with various other prostaglandin receptors, it mediates the effects of prostaglandin F(2-alpha) which include modulation of intraocular pressure, corpus luteum regression, and possibly uterine smooth muscle contraction. Alternative mRNA splicing gives rise to two isoforms, FP(A) and FP(B), that differ in their intracellular carboxyl termini and result in different mechanisms for receptor internalization. Prostaglandin F2-alpha receptor expression has been documented in eye, ovary, placenta, and uterus. ESTs have been isolated from placental libraries.

Long Name

Prostaglandin F Receptor

Alternate Names

FP, PGF2-alpha Receptor

Entrez Gene IDs

5737 (Human)

Gene Symbol

PTGFR

Additional PTGFR Products

Product Documents for PTGFR Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PTGFR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PTGFR Antibody - BSA Free

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Protocols

View specific protocols for PTGFR Antibody - BSA Free (NLS1049):

Immunohistochemistry Protocol for Prostaglandin F2 alpha Receptor Antibody (NLS1049):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4-um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene approximately 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol approximately 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol approximately 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol approximately 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water approximately 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block approximately 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody approximately 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T approximately 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary approximately 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
17. Apply an alkaline phosphatase steptavidin approximately 30 minutes at RT.
18. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
19. Apply an alkaline phosphatase chromagen substrate approximately 30 minutes at RT.
20. Rinse the slide in distilled water approximately 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol approximately 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol approximately 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol approximately 1 minute each @ RT.
24. Wash the slides in 3 changes of xyleneapproximately 1 minute each @ RT.
25. Apply cover slip.

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FAQs

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