PTP1B/PTPN1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87191

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PTP1B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RFSYLAVIEGAKFIMGDSSVQDQWKELSHEDLEPPPEHIPPPPRPPKRILEPHNGKCREFFPNHQWVKEETQEDKDCPIKEEKGSPLNAAPYGIESMSQDTEVRSRVVGGSLRGAQAASPAKGEPSLPEKDEDHALSYW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTP1B/PTPN1 Antibody - BSA Free

Western Blot: PTP1B/PTPN1 Antibody [NBP1-87191]

Western Blot: PTP1B/PTPN1 Antibody [NBP1-87191]

Western Blot: PTP1B/PTPN1 Antibody [NBP1-87191] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PTPN1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody [NBP1-87191]

Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody [NBP1-87191]

Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody [NBP1-87191] - Staining of human cell line U-2 OS shows positivity in endoplasmic reticulum.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191]

Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody [NBP1-87191] - Staining of human lymph node shows moderate to strong cytoplasmic positivity in non-germinal center cells.

Applications for PTP1B/PTPN1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTP1B

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Gene Symbol

PTPN1

Additional PTP1B Products

Product Documents for PTP1B/PTPN1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PTP1B/PTPN1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

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Protocols

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