PTPN12 Antibody (BL-5-2F8)

Novus Biologicals | Catalog # NBP2-76395

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-5-2F8
View all PTPN12 Primary Antibodies »

Product Specifications

Immunogen

Immunogen sequence between amino acids 499 and 780

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTPN12 Antibody (BL-5-2F8)

Western Blot: PTPN12 Antibody (BL-5-2F8) [NBP2-76395]

Western Blot: PTPN12 Antibody (BL-5-2F8) [NBP2-76395]

Western Blot: PTPN12 Antibody (BL-5-2F8) [NBP2-76395] - Detection of human PTPN12 by WB of HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 [SK], and HCT 116 lysate. Antibody: Rabbit anti-PTPN12 recombinant monoclonal [BL-5-2F8] (NBP2-76395). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry/ Immunofluorescence: PTPN12 Antibody (BL-5-2F8) [NBP2-76395]

Immunocytochemistry/ Immunofluorescence: PTPN12 Antibody (BL-5-2F8) [NBP2-76395]

Immunocytochemistry/Immunofluorescence: PTPN12 Antibody (BL-5-2F8) [NBP2-76395] - Detection of human PTPN12 in FFPE SW620 cells by ICC. Antibody: Rabbit anti-PTPN12 recombinant monoclonal [BL-5-2F8] (NBP2-76395). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry: PTPN12 Antibody (BL-5-2F8) [NBP2-76395]

Immunohistochemistry: PTPN12 Antibody (BL-5-2F8) [NBP2-76395]

Immunohistochemistry: PTPN12 Antibody (BL-5-2F8) [NBP2-76395] - Detection of human PTPN12 in FFPE non-small cell lung carcinoma by IHC. Antibody: Rabbit anti-PTPN12 recombinant monoclonal [BL-5-2F8] (NBP2-76395). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry: PTPN12 Antibody (BL-5-2F8) [NBP2-76395]

Immunohistochemistry: PTPN12 Antibody (BL-5-2F8) [NBP2-76395]

Immunohistochemistry: PTPN12 Antibody (BL-5-2F8) [NBP2-76395] - Detection of human PTPN12 in FFPE mammary gland by IHC. Antibody: Rabbit anti-PTPN12 recombinant monoclonal [BL-5-2F8] (NBP2-76395). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunoprecipitation: PTPN12 Antibody (BL-5-2F8) [NBP2-76395]

Immunoprecipitation: PTPN12 Antibody (BL-5-2F8) [NBP2-76395]

Immunoprecipitation: PTPN12 Antibody (BL-5-2F8) [NBP2-76395] - Detection of human PTPN12 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-PTPN12 recombinant monoclonal [BL-5-2F8] (NBP2-76395) and another rabbit anti-PTPN12 antibody.

Applications for PTPN12 Antibody (BL-5-2F8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:200

Immunohistochemistry

1:20 - 1:200

Immunoprecipitation

20 ul/0.5 mg lysate

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: PTPN12

PTPN12 is a cytosolic protein tyrosine phosphatase (PTP) that contains a C-terminal PEST motif and plays an important role in cytoskeletal organization, immune cell activation, apoptosis, and embryonic development. PTPN12 appears to contribute to these cellular processes through its interaction with a variety of signaling and cytoskeletal-associated proteins. The recent discovery that PTPN12 is modulated by caspase-3 cleavage has led to the analysis of the role of PTPN12 and other PTPs as regulators of apoptosis.

Long Name

Tyrosine-protein phosphatase non-receptor type 12

Alternate Names

PTP-PEST, PTPG1

Gene Symbol

PTPN12

Additional PTPN12 Products

Product Documents for PTPN12 Antibody (BL-5-2F8)

Certificate of Analysis

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Product Specific Notices for PTPN12 Antibody (BL-5-2F8)

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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