PTPN14/PTPD2 Antibody (2C12E3) - BSA Free

Novus Biologicals | Catalog # NBP2-61810

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2C12E3

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human PTPN14/PTPD2 (AA: 896-1169) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

135.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTPN14/PTPD2 Antibody (2C12E3) - BSA Free

Western Blot: PTPN14/PTPD2 Antibody (2C12E3)BSA Free [NBP2-61810]

Western Blot: PTPN14/PTPD2 Antibody (2C12E3)BSA Free [NBP2-61810]

Western Blot: PTPN14/PTPD2 Antibody (2C12E3) [NBP2-61810] - Analysis using PTPN14 mAb against human PTPN14 (AA: 896-1169) recombinant protein. (Expected MW is 57.5 kDa)
Immunocytochemistry/ Immunofluorescence: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

Immunocytochemistry/ Immunofluorescence: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

Immunocytochemistry/Immunofluorescence: PTPN14/PTPD2 Antibody (2C12E3) [NBP2-61810] - Analysis of Hela cells using PTPN14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

Immunohistochemistry-Paraffin: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

Immunohistochemistry-Paraffin: PTPN14/PTPD2 Antibody (2C12E3) [NBP2-61810] - Endometrial cancer tissues using PTPN14 mouse mAb with DAB staining.
Flow Cytometry: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

Flow Cytometry: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

Flow Cytometry: PTPN14/PTPD2 Antibody (2C12E3) [NBP2-61810] - Analysis of Hela cells using PTPN14 mouse mAb (green) and negative control (red).
Western Blot: PTPN14/PTPD2 Antibody (2C12E3)BSA Free [NBP2-61810]

Western Blot: PTPN14/PTPD2 Antibody (2C12E3)BSA Free [NBP2-61810]

Western Blot: PTPN14/PTPD2 Antibody (2C12E3) [NBP2-61810] - Analysis using PTPN14 mAb against HEK293 (1) and PTPN14 (AA: 896-1169)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

Immunocytochemistry/ Immunofluorescence: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

Immunocytochemistry/Immunofluorescence: PTPN14/PTPD2 Antibody (2C12E3) [NBP2-61810] - Analysis of Hela cells using PTPN14 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunohistochemistry-Paraffin: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

Immunohistochemistry-Paraffin: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

Immunohistochemistry-Paraffin: PTPN14/PTPD2 Antibody (2C12E3) [NBP2-61810] - Analysis of rectum cancer tissues using PTPN14 mouse mAb with DAB staining.
ELISA: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

ELISA: PTPN14/PTPD2 Antibody (2C12E3) - BSA Free [NBP2-61810]

ELISA: PTPN14/PTPD2 Antibody (2C12E3) [NBP2-61810] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for PTPN14/PTPD2 Antibody (2C12E3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTPN14

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an N-terminal noncatalytic domain similar to that of band 4.1 superfamily cytoskeleton-associated proteins, which suggested the membrane or cytoskeleton localization of this protein. It appears to regulate lymphatic development in mammals, and a loss of function mutation has been found in a kindred with a lymphedema-choanal atresia.;

Long Name

Protein Tyrosine Phosphatase Non-receptor Type 14

Alternate Names

PEZ

Gene Symbol

PTPN14

Additional PTPN14 Products

Product Documents for PTPN14/PTPD2 Antibody (2C12E3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PTPN14/PTPD2 Antibody (2C12E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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FAQs

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