PTPRN2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35468

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PTPRN2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-242 of human PTPRN2 (NP_570858.2).

Sequence:
AAPSSVPRGRQLPGRLGCLLEEGLCGASEACVNDGVFGRCQKVPAMDFYRYEVSPVALQRLRVALQKLSGTGFTWQDDYTQYVMDQELADLPKTYLRRPEASSPARPSKHSVGSERRYSREGGAALANALRRHLPFLEALSQAPASDVLARTHTAQDRPPAEGDDRFSESILTYVAHTSALTYPPGSRTQLREDLLPRTLGQLQPDELSPKVDSGVDRHHL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

111 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTPRN2 Antibody - BSA Free

PTPRN2 Antibody

Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] -

Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using PTPRN2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
PTPRN2 Antibody

Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] -

Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using PTPRN2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
PTPRN2 Antibody

Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] -

Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] - Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using PTPRN2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
PTPRN2 Antibody

Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] -

Immunohistochemistry: PTPRN2 Antibody [NBP3-35468] - Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using PTPRN2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
PTPRN2 Antibody

Western Blot: PTPRN2 Antibody [NBP3-35468] -

Western Blot: PTPRN2 Antibody [NBP3-35468] - Western blot analysis of various lysates using PTPRN2 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

Applications for PTPRN2 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunohistochemistry-Paraffin

1:50 - 1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTPRN2

PTPRN2 is encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domain, and thus represents a receptor-type PTP. The catalytic domain of this PTP is most closely related to PTPRN/IA-2beta. This PTP and PTPRN are both found to be major autoantigens associated with insulin-dependent diabetes mellitus. Three alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.

Alternate Names

EC 3.1.3.48, IA-2beta, IAR/receptor-like protein-tyrosine phosphatase, ICAARIARPTPRP, Islet cell autoantigen-related protein, KIAA0387, Phogrin, protein tyrosine phosphatase receptor pi, protein tyrosine phosphatase, receptor type, N polypeptide 2, receptor-type tyrosine-protein phosphatase N2, R-PTP-N2, tyrosine phosphatase IA-2 beta

Gene Symbol

PTPRN2

Additional PTPRN2 Products

Product Documents for PTPRN2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PTPRN2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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