![Western Blot: Punt Antibody [NB300-662]](https://resources.rndsystems.com/images/products/Punt-Antibody-Western-Blot-NB300-662-img0001.jpg "Western Blot: Punt Antibody [NB300-662]")
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Key Product Details
Species Reactivity
Drosophila
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Synthetic peptide: KSTTEATTQVPKEKTQDGSN[C]. Peptides was amidated for conjugation.
Reactivity Notes
Fruit fly.
Specificity
Punt
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Punt Antibody
Western Blot: Punt Antibody [NB300-662]
Western Blot: Punt Antibody [NB300-662] - Detection of Punt at a dilution of 1:200. Rows A and C contain Punt-Flag constructs.Applications for Punt Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:200
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
Immunocytochemistry, Immunoprecipitation, Western Blot Detects a band of approximately 63 kDa( Optimal dilutions and concentrations should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.
Preservative
0.02% Sodium Azide
Concentration
0.75 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Punt
Alternate Names
Activin A receptor 88CD, Activin receptor, Activin receptor type II, ATR, Atr II, Atr88CD, AtrII, CG7904, dlhC, Dorsal holes C, j5A5, l(3)10460, Pun, Punt receptor serine/threonine kinase, Put, STK C, STKC, TGF B, TGF beta, TGF beta l, TGF beta type receptor like, Tgf r, TGFB, TGFbeta
Additional Punt Products
Product Documents for Punt Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Punt Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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