PYCRL Antibody (4F11) - Azide and BSA Free

Novus Biologicals | Catalog # H00065263-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4F11

Format

Azide and BSA Free
View all PYCRL Primary Antibodies »

Product Specifications

Immunogen

PYCRL (NP_075566, 32 a.a. ~ 131 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCLLVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVV

Reactivity Notes

Other species not tested.

Specificity

PYCRL (4F11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PYCRL Antibody (4F11) - Azide and BSA Free

Western Blot: PYCRL Antibody (4F11) [H00065263-M01]

Western Blot: PYCRL Antibody (4F11) [H00065263-M01]

Western Blot: PYCRL Antibody (4F11) [H00065263-M01] - PYCRL monoclonal antibody (M01), clone 4F11 Analysis of PYCRL expression in HeLa.
Immunocytochemistry/ Immunofluorescence: PYCRL Antibody (4F11) [H00065263-M01]

Immunocytochemistry/ Immunofluorescence: PYCRL Antibody (4F11) [H00065263-M01]

Immunocytochemistry/Immunofluorescence: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of monoclonal antibody to PYCRL on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: PYCRL Antibody (4F11) [H00065263-M01]

Western Blot: PYCRL Antibody (4F11) [H00065263-M01]

Western Blot: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of PYCRL expression in transfected 293T cell line by PYCRL monoclonal antibody (M01), clone 4F11.Lane 1: PYCRL transfected lysate (Predicted MW: 28.6 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: PYCRL Antibody (4F11) [H00065263-M01]

Immunoprecipitation: PYCRL Antibody (4F11) [H00065263-M01]

Immunoprecipitation: PYCRL Antibody (4F11) [H00065263-M01] - Analysis of PYCRL transfected lysate using anti-PYCRL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PYCRL monoclonal antibody.
ELISA: PYCRL Antibody (4F11) [H00065263-M01]

ELISA: PYCRL Antibody (4F11) [H00065263-M01]

ELISA: PYCRL Antibody (4F11) [H00065263-M01] - Detection limit for recombinant GST tagged PYCRL is approximately 0.3ng/ml as a capture antibody.

Applications for PYCRL Antibody (4F11) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PYCRL

Alternate Names

EC 1.5.1.2, FLJ13852, P5C reductase 3, P5CR 3, pyrroline-5-carboxylate reductase 3, pyrroline-5-carboxylate reductase-like, Pyrroline-5-carboxylate reductase-like protein

Entrez Gene IDs

65263 (Human)

Gene Symbol

PYCR3

UniProt

NP_075566 (Human)

Additional PYCRL Products

Product Documents for PYCRL Antibody (4F11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PYCRL Antibody (4F11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PYCRL Antibody (4F11) - Azide and BSA Free

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Protocols

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FAQs

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