PYGL Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86147

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PYGL Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PYGL Antibody - BSA Free

PYGL Antibody - BSA Free Western Blot: PYGL Antibody - BSA Free [NBP1-86147]

Western Blot: PYGL Antibody - BSA Free [NBP1-86147]

Analysis in human cell lines PC-3 and SK-MEL-30 using Anti-PYGL antibody. Corresponding PYGL RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Analysis in human liver and pancreas tissues. Corresponding PYGL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human pancreas shows very weak positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.
Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human cerebral cortex shows very weak positivity in neuronal cells.
Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human testis shows very weak positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147]

Immunohistochemistry-Paraffin: PYGL Antibody [NBP1-86147] - Staining of human bone marrow, cerebral cortex, liver and testis using Anti-PYGL antibody (A) NBP1-86147 shows similar protein distribution across tissues to independent antibody NBP1-86182 (B).
PYGL Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: PYGL Antibody - BSA Free [NBP1-86147]

Immunocytochemistry/ Immunofluorescence: PYGL Antibody - BSA Free [NBP1-86147]

Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Applications for PYGL Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PYGL

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Activity of phosphorylase is controlled both by allosteric means (through the noncovalent binding of metabolites) and by covalent modification. Thus AMP allosterically activates, whereas ATP, ADP, and glucose 6 phosphate allosterically inhibit, phosphorylase B.

Long Name

Glycogen phosphorylase, liver form

Alternate Names

EC 2.4.1.1, glycogen phosphorylase, liver form, GSD6, phosphorylase, glycogen, liver, phosphorylase, glycogen; liver

Gene Symbol

PYGL

Additional PYGL Products

Product Documents for PYGL Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PYGL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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