Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) - Azide and BSA Free

Novus Biologicals | Catalog # H00005162-M03

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2B2

Format

Azide and BSA Free
View all Pyruvate Dehydrogenase E1 beta subunit Primary Antibodies »

Product Specifications

Immunogen

PDHB (NP_000916, 250 a.a. ~ 359 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LEAAAVLSKEGVECEVINMRTIRPMDMETIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKILEDNSIPQVKDIIFAIKKTLNI

Specificity

PDHB - pyruvate dehydrogenase (lipoamide) beta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03]

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03]

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of PDHB expression in transfected 293T cell line by PDHB monoclonal antibody (M03), clone 2B2. Lane 1: PDHB transfected lysatE (39.2 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03]

Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of monoclonal antibody to PDHB on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml
Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03]

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03]

Western Blot: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Analysis of PDHB expression in HeLa (Cat # L013V1).
ELISA: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03]

ELISA: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03]

ELISA: Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) [H00005162-M03] - Detection limit for recombinant GST tagged PDHB is approximately 1ng/ml as a capture antibody.

Applications

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Pyruvate Dehydrogenase E1 beta subunit

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)

Alternate Names

DKFZp564K0164, mitochondrial, pyruvate dehydrogenase (lipoamide) beta, pyruvate dehydrogenase, E1 beta polypeptide

Entrez Gene IDs

5162 (Human)

Gene Symbol

PDHB

OMIM

179060 (Human)

UniProt

NP_000916 (Human)

Additional Pyruvate Dehydrogenase E1 beta subunit Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) - Azide and BSA Free

Customer Reviews

There are currently no reviews for this product. Be the first to review Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) - Azide and BSA Free and earn rewards!

Have you used Pyruvate Dehydrogenase E1 beta subunit Antibody (2B2) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies