Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11)
Novus Biologicals | Catalog # NBP2-22171
![Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11)BSA Free [NBP2-22171]](https://resources.rndsystems.com/images/products/PDK-1-Antibody-4A11-Western-Blot-NBP2-22171-img0004.jpg "Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11)BSA Free [NBP2-22171]")
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Key Product Details
Species Reactivity
Validated:
Human, Rat, Monkey
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4A11
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Product Specifications
Immunogen
Purified recombinant fragment of human Pyruvate Dehydrogenase Kinase 1/PDK1 expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11)
Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11)BSA Free [NBP2-22171]
Western Blot: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) - BSA Free [NBP2-22171]](https://resources.rndsystems.com/images/products/PDK-1-Antibody-4A11-Immunocytochemistry-Immunofluorescence-NBP2-22171-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) - BSA Free [NBP2-22171]")
Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) - BSA Free [NBP2-22171]
Immunocytochemistry/Immunofluorescence: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) - BSA Free [NBP2-22171]](https://resources.rndsystems.com/images/products/PDK-1-Antibody-4A11-Immunohistochemistry-Paraffin-NBP2-22171-img0003.jpg "Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) - BSA Free [NBP2-22171]")
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) - BSA Free [NBP2-22171]
Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of breast cancer tissues (left) and brain tissues (right) using PDK1 mouse mAb with DAB staining.![Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) - BSA Free [NBP2-22171]](https://resources.rndsystems.com/images/products/PDK-1-Antibody-4A11-Flow-Cytometry-NBP2-22171-img0001.jpg "Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) - BSA Free [NBP2-22171]")
Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) - BSA Free [NBP2-22171]
Flow Cytometry: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11) [NBP2-22171] - Analysis of Lovo cells using PDK1 mouse mAb (green) and negative control (purple).Applications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11)
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Reviewed Applications
Read 1 review rated 3 using NBP2-22171 in the following applications:
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
Ascites
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Pyruvate Dehydrogenase Kinase 1/PDK1
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.
Alternate Names
EC 2.7.11, EC 2.7.11.2, mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1, PDH kinase 1, PDK1, PDK-1, PKB kinase, Pyruvate dehydrogenase kinase isoform 1, pyruvate dehydrogenase kinase, isoenzyme 1, pyruvate dehydrogenase kinase, isozyme 1, Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial
Gene Symbol
PDK1
Additional Pyruvate Dehydrogenase Kinase 1/PDK1 Products
Product Documents for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (4A11)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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