Key Product Details

Species Reactivity

Rabbit

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
Loading...

Product Specifications

Immunogen

Pyruvate Kinase (Rabbit Muscle) (Uniprot: P11974)

Reactivity Notes

Cross-reactivity against pyruvate kinase from other mammalian tissues is expected but has not been specifically determined.

Specificity

This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross-reactivity against pyruvate kinase from other mammalian tissues is expected but has not been specifically determined.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Pyruvate Kinase (Rabbit Muscle)

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Pyruvate Kinase Antibody - BSA Free

Western Blot: Pyruvate Kinase Antibody [NBP2-44231]

Western Blot: Pyruvate Kinase Antibody [NBP2-44231]

Western Blot: Pyruvate Kinase Antibody [NBP2-44231] - Western Blot of Goat Pyruvate Kinase antibody. Lane 1: CHO K1 Lysate [10ug].Lane 2: CHO S Lysate [10ug]. Lane 3: Opal Pre-stained Molecular Weight Marker. Primary Antibody: Anti-Pyruvate Kinase at 1:50 overnight at 2-8C. Secondary Antibody: Donkey Anti-Goat IgG CY5 conjugate at 1:10,000 for 30 mins at RT.Expect: ~57kDa.

Applications for Pyruvate Kinase Antibody - BSA Free

Application
Recommended Usage

ELISA

1:4000-1:20000

Immunohistochemistry

1:500-1:2000

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested in western blot. This antibody is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Pyruvate Kinase

Alternate Names

PKM, PKM2

UniProt

Additional Pyruvate Kinase Products

Product Documents for Pyruvate Kinase Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Pyruvate Kinase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Pyruvate Kinase Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Pyruvate Kinase Antibody - BSA Free and earn rewards!

Have you used Pyruvate Kinase Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies
Loading...