Rab27a Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83327

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rab27a Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLTNEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYFETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rab27a Antibody - BSA Free

Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327]

Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327]

Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327] - Analysis in human stomach and skeletal muscle tissues. Corresponding RAB27A RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327]

Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327]

Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327]

Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327]

Immunohistochemistry-Paraffin: Rab27a Antibody [NBP1-83327] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells with additional stron membranous positivity in a subset of glandular cells.
Rab27a Antibody - BSA Free Immunohistochemistry-Paraffin: Rab27a Antibody - BSA Free [NBP1-83327]

Immunohistochemistry-Paraffin: Rab27a Antibody - BSA Free [NBP1-83327]

Staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Rab27a Antibody - BSA Free Western Blot: Rab27a Antibody - BSA Free [NBP1-83327]

Western Blot: Rab27a Antibody - BSA Free [NBP1-83327]

Analysis in human cell lines SK-MEL-30 and Caco-2 using Anti-RAB27A antibody. Corresponding RAB27A RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Applications for Rab27a Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rab27a

RAB27A is encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.

Long Name

RAs Genes from Brain Protein 27A

Alternate Names

GS2, HsT18676, RAM

Gene Symbol

RAB27A

Additional Rab27a Products

Product Documents for Rab27a Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Rab27a Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy
Lysosomal Enzymes

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Protocols

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FAQs

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