Human Survivin Antibody

(36 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Survivin. This antibody also detects an unidentified ~51 kDa protein band.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Survivin
    aa 1-142
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunohistochemistry
    3-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Survivin by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Survivin at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Immunohistochemistry
Survivin in Human Breast Cancer Tissue. Survivin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Immunohistochemistry
Survivin in Human Prostate Cancer Tissue. Survivin was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 10 µg/mL Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Survivin by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 23 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Survivin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF886). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Survivin
Survivin also known as Apoptosis inhibitor survivin and Baculoviral IAP repeat-containing protein 5 (BIRC5) is an 18 kDa member of the inhibitor of apoptosis (IAP) gene family, which encodes negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple Baculovirus IAP repeat (BIR) domains, but this gene encodes seven isoforms with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Survivin is expressed in a cell cycle-dependent manner and associates with the mitotic apparatus.
  • Entrez Gene IDs:
    332 (Human)
  • Alternate Names:
    API4 survivin variant 3 alpha; API4; Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; BIRC5; EPR-1; IAP4; Survivin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Epigenetic silencing of XAF1 in high-grade gliomas is associated with IDH1 status and improved clinical outcome
    Authors: TR Reich, OJ Switzeny, M Renovanz, C Sommer, B Kaina, M Christmann, MT Tomicic
    Oncotarget, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study
    Onco Targets Ther, 2016;9(0):2601-13.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma
    Authors: Katharina Engel
    EMBO Mol Med, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  4. Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies
    Molecules, 2016;21(8):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Sulindac sulfide inhibits colon cancer cell growth and downregulates specificity protein transcription factors.
    Authors: Li X, Pathi S, Safe S
    BMC Cancer, 2015;15(0):974.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. STAT3 activation is required for the antiapoptotic effects of prolactin in cervical cancer cells.
    Authors: Ramirez de Arellano A, Lopez-Pulido E, Martinez-Neri P, Estrada Chavez C, Gonzalez Lucano R, Fafutis-Morris M, Aguilar-Lemarroy A, Munoz-Valle J, Pereira-Suarez A
    Cancer Cell Int, 2015;15(0):83.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Stage-dependent prognostic impact of molecular signatures in clear cell renal cell carcinoma.
    Authors: Weber T, Meinhardt M, Zastrow S, Wienke A, Erdmann K, Hofmann J, Fuessel S, Wirth M
    Onco Targets Ther, 2014;7(0):645-54.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  8. Small-molecule survivin inhibitor YM155 enhances radiosensitization in esophageal squamous cell carcinoma by the abrogation of G2 checkpoint and suppression of homologous recombination repair.
    Authors: Qin Q, Cheng H, Lu J, Zhan L, Zheng J, Cai J, Yang X, Xu L, Zhu H, Zhang C, Liu J, Ma J, Zhang X, Dai S, Sun X
    J Hematol Oncol, 2014;7(0):62.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Survivin safeguards chromosome numbers and protects from aneuploidy independently from p53.
    Authors: Wiedemuth R, Klink B, Topfer K, Schrock E, Schackert G, Tatsuka M, Temme A
    Mol Cancer, 2014;13(0):107.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  10. Survivin expression promotes VEGF-induced tumor angiogenesis via PI3K/Akt enhanced beta-catenin/Tcf-Lef dependent transcription.
    Authors: Fernandez J, Rodriguez D, Valenzuela M, Calderon C, Urzua U, Munroe D, Rosas C, Lemus D, Diaz N, Wright M, Leyton L, Tapia J, Quest A
    Mol Cancer, 2014;13(0):209.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Sequence-dependent combination therapy with doxorubicin and a survivin-specific small interfering RNA nanodrug demonstrates efficacy in models of adenocarcinoma.
    Authors: Ghosh S, Yigit M, Uchida M, Ross A, Barteneva N, Moore A, Medarova Z
    Int J Cancer, 2014;134(7):1758-66.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. Protein kinase C zeta regulates survivin expression and inhibits apoptosis in colon cancer.
    Authors: Umemori Y, Kuribayashi K, Nirasawa S, Kondoh T, Tanaka M, Kobayashi D, Watanabe N
    Int J Oncol, 2014;45(3):1043-50.
    Species: Human
    Sample Type: Protein
    Application: WB
  13. The pterocarpanquinone LQB-118 induces apoptosis in acute myeloid leukemia cells of distinct molecular subtypes and targets FoxO3a and FoxM1 transcription factors.
    Authors: Nestal de Moraes G, Castro C, Salustiano E, Dumas M, Costas F, Lam E, Costa P, Maia R
    Int J Oncol, 2014;45(5):1949-58.
    Species: Human
    Sample Type: Protein
    Application: WB
  14. COX-2-independent effects of celecoxib sensitize lymphoma B cells to TRAIL-mediated apoptosis.
    Authors: Gallouet A, Travert M, Bresson-Bepoldin L, Guilloton F, Pangault C, Caulet-Maugendre S, Lamy T, Tarte K, Guillaudeux T
    Clin Cancer Res, 2014;20(10):2663-73.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
    Authors: Hage C, Rausch V, Giese N, Giese T, Schonsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I
    Cell Death Dis, 2013;4(0):e627.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  16. Smac mimetic sensitizes glioblastoma cells to Temozolomide-induced apoptosis in a RIP1- and NF-kappaB-dependent manner.
    Authors: Wagner L, Marschall V, Karl S, Cristofanon S, Zobel K, Deshayes K, Vucic D, Debatin K, Fulda S
    Oncogene, 2013;32(8):988-97.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  17. Azadirone, a limonoid tetranortriterpene, induces death receptors and sensitizes human cancer cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) through a p53 protein-independent mechanism: evidence for the role of the ROS-ERK-CHOP-death receptor pathway.
    Authors: Gupta, Subash C, Francis, Sajin K, Nair, Mangalam, Mo, Yin-Yuan, Aggarwal, Bharat B
    J Biol Chem, 2013;288(45):32343-56.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  18. Phenotypic characterization of hereditary epithelial ovarian cancer based on a tissue microarray study.
    Authors: Munoz-Repeto I, Garcia M, Kamieniak M, Ramon Y Cajal T, Domingo S, Cazorla A, Garcia Donas J, Hernando Polo S, Garcia Sagredo J, Hernandez E, Lacambra C, Saez R, Robles L, Borrego S, Prat J, Palacios J, Benitez J
    Histol Histopathol, 2013;28(1):133-44.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  19. Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
    Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G
    PLoS ONE, 2012;7(9):e45492.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  20. RIP1 is required for IAP inhibitor-mediated sensitization of childhood acute leukemia cells to chemotherapy-induced apoptosis.
    Authors: Loder S, Fakler M, Schoeneberger H, Cristofanon S, Leibacher J, Vanlangenakker N, Bertrand MJ, Vandenabeele P, Jeremias I, Debatin KM, Fulda S
    Leukemia, 2011;26(5):1020-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  21. Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus.
    Authors: Toosi, Siavash, Habib, Nancy, Torres, Geneviev, Reynolds, Sandra R, Bystryn, Jean-Cla
    J Invest Dermatol, 2011;131(11):2327-9.
    Species: Human
    Sample Type: Serum
    Application: WB
  22. Myricetin sensitizes malignant glioma cells to TRAIL-mediated apoptosis by down-regulation of the short isoform of FLIP and bcl-2.
    Authors: Siegelin MD, Gaiser T, Habel A, Siegelin Y
    Cancer Lett., 2009;283(2):230-8.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  23. SOX7, down-regulated in colorectal cancer, induces apoptosis and inhibits proliferation of colorectal cancer cells.
    Authors: Zhang Y, Huang S, Dong W, Li L, Feng Y, Pan L, Han Z, Wang X, Ren G, Su D, Huang B, Lu J
    Cancer Lett., 2009;277(1):29-37.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  24. Cell polarization during monopolar cytokinesis.
    Authors: Hu CK, Coughlin M, Field CM, Mitchison TJ
    J. Cell Biol., 2008;181(2):195-202.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  25. Regulation of apoptosis by XIAP ubiquitin-ligase activity.
    Authors: Schile AJ, Garcia-Fernandez M, Steller H
    Genes Dev., 2008;22(16):2256-66.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  26. Nuclear localization of Survivin renders HeLa tumor cells more sensitive to apoptosis by induction of p53 and Bax.
    Authors: Temme A, Rodriguez JA, Hendruschk S, Gunes S, Weigle B, Schakel K, Schmitz M, Bachmann M, Schackert G, Rieber EP
    Cancer Lett., 2007;250(2):177-93.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  27. Survivin in the human hair follicle.
    Authors: Botchkareva NV, Kahn M, Ahluwalia G, Shander D
    J. Invest. Dermatol., 2007;127(2):479-82.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  28. Mitogen-activated protein kinase kinase inhibition enhances nuclear proapoptotic function of p53 in acute myelogenous leukemia cells.
    Authors: Kojima K, Konopleva M, Samudio IJ
    Cancer Res., 2007;67(7):3210-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  29. Indirubin-3'-monoxime, a CDK inhibitor induces growth inhibition and apoptosis-independent up-regulation of survivin in transitional cell cancer.
    Authors: Perabo FG, Frossler C, Landwehrs G, Schmidt DH, von Rucker A, Wirger A, Muller SC
    Anticancer Res., 2006;26(3):2129-35.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  30. cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis.
    Authors: Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU
    Eur. J. Immunol., 2006;36(7):1975-84.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  31. Survivin interacts with Smac/DIABLO in ovarian carcinoma cells but is redundant in Smac-mediated apoptosis.
    Authors: McNeish IA, Lopes R, Bell SJ, McKay TR, Fernandez M, Lockley M, Wheatley SP, Lemoine NR
    Exp. Cell Res., 2005;302(1):69-82.
    Species: Human
    Sample Type: Cell Lysates
    Application: Affinity Chromatography
  32. The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly.
    Authors: Sampath SC, Ohi R, Leismann O, Salic A, Pozniakovski A, Funabiki H
    Cell, 2004;118(2):187-202.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  33. Inflammation-associated cell cycle-independent block of apoptosis by survivin in terminally differentiated neutrophils.
    Authors: Altznauer F, Martinelli S, Yousefi S, Thurig C, Schmid I, Conway EM, Schoni MH, Vogt P, Mueller C, Fey MF, Zangemeister-Wittke U, Simon HU
    J. Exp. Med., 2004;199(10):1343-54.
    Species: Human
    Sample Type: Whole Cells
    Application: WB
  34. Apoptotic signaling pathways induced by nitric oxide in human lymphoblastoid cells expressing wild-type or mutant p53.
    Authors: Li CQ, Robles AI, Hanigan CL, Hofseth LJ, Trudel LJ, Harris CC, Wogan GN
    Cancer Res., 2004;64(9):3022-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  35. Elevated expression level of survivin protein in soft-tissue sarcomas is a strong independent predictor of survival.
    Authors: Kappler M, Kotzsch M, Bartel F, Fussel S, Lautenschlager C, Schmidt U, Wurl P, Bache M, Schmidt H, Taubert H, Meye A
    Clin. Cancer Res., 2003;9(3):1098-104.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  36. Localization, dynamics, and function of survivin revealed by expression of functional survivinDsRed fusion proteins in the living cell.
    Authors: Temme A, Rieger M, Reber F, Lindemann D, Weigle B, Diestelkoetter-Bachert P, Ehninger G, Tatsuka M, Terada Y, Rieber EP
    Mol. Biol. Cell, 2003;14(1):78-92.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 36 Citations
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