Human/Mouse/Rat HSP70/HSPA1A Antibody
Human/Mouse/Rat HSP70/HSPA1A Antibody Summary
Met1-Asp641
Accession # P08107
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human, Mouse, and Rat HSP70/HSPA1A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, L-929 mouse fibroblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HSP70/HSPA1A at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
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Detection of Human HSP70/HSPA1A by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with heat shocked (HS), loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 66 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Detection of Human and Mouse HSP70/HSPA1A by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP70/HSPA1A at approximately 66 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat HSP70/HSPA1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1663). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSP70/HSPA1A
Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up‑regulated in response to cellular stress. HSP70 is a 72 kDa member of the heat shock protein 70 family of proteins. HSP70, also known as HSPA1A, HSP70-1, and HSP72 is a 641 amino acid (aa) heat shock protein. Over aa 1-641, human HSP70 shares 95% and 97% aa identity to mouse and rat HSP70.
Product Datasheets
Citations for Human/Mouse/Rat HSP70/HSPA1A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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The oncogenic transcription factor FOXQ1 is a differential regulator of Wnt target genes
Authors: G Pizzolato, L Moparthi, S Söderholm, C Cantù, S Koch
Journal of Cell Science, 2022;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Plasma extracellular vesicles bearing PD-L1, CD40, CD40L or TNF-RII are significantly reduced after treatment of AIDS-NHL
Authors: LE Martínez, S Lensing, D Chang, LI Magpantay, R Mitsuyasu, RF Ambinder, JA Sparano, O Martínez-M, M Epeldegui
Oncogene, 2022;12(1):9185.
Species: Human
Sample Types: Extracellular Vesicle Lysate
Applications: Western Blot -
Acute Ethanol Exposure during Synaptogenesis Rapidly Alters Medium Spiny Neuron Morphology and Synaptic Protein Expression in the Dorsal Striatum
Authors: E Clabough, J Ingersoll, T Reekes, A Gleichsner, A Ryan
International Journal of Molecular Sciences, 2021;23(1):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Long-Term Low-Dose Delta-9-Tetrahydrocannbinol (THC) Administration to Simian Immunodeficiency Virus (SIV) Infected Rhesus Macaques Stimulates the Release of Bioactive Blood Extracellular Vesicles (EVs) that Induce Divergent Structural Adaptations and Signaling Cues
Authors: Y Lyu, S Kopcho, M Mohan, CM Okeoma
Cells, 2020;9(10):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease
Authors: H Gerber, S Mosser, B Boury-Jamo, M Stumpe, A Piersigill, C Goepfert, J Dengjel, U Albrecht, F Magara, PC Fraering
Acta Neuropathol Commun, 2019;7(1):13.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Augmentation of vaccine-induced humoral and cellular immunity by a physical radiofrequency adjuvant
Authors: Y Cao, X Zhu, MN Hossen, P Kakar, Y Zhao, X Chen
Nat Commun, 2018;9(1):3695.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
A role for heat shock factor 1 in hypercapnia-induced inhibition of inflammatory cytokine expression
Authors: Z Lu, SM Casalino-M, A Nair, A Buchbinder, GRS Budinger, PHS Sporn, KL Gates
FASEB J., 2018;0(0):fj201701164R.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Substance P acts via the neurokinin receptor 1 to elicit bronchoconstriction, oxidative stress, and upregulated ICAM-1 expression after oil smoke exposure.
Authors: Li PC, Chen WC, Chang LC, Lin SC
Am. J. Physiol. Lung Cell Mol. Physiol., 2008;294(5):L912-20.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot
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