Rad51C Antibody (2H11/6) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80923
Key Product Details
Species Reactivity
Human, Mouse, Primate, Yeast
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2H11/6
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502]
Localization
Nuclear
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rad51C Antibody (2H11/6) - Azide and BSA Free
![Western Blot: Rad51C Antibody (2H11/6)Azide and BSA Free [NBP2-80923]](https://resources.rndsystems.com/images/products/Rad51C-Antibody-2H11-6-Azide-and-BSA-Free-Western-Blot-NBP2-80923-img0002.jpg "Western Blot: Rad51C Antibody (2H11/6)Azide and BSA Free [NBP2-80923]")
Western Blot: Rad51C Antibody (2H11/6)Azide and BSA Free [NBP2-80923]
Western Blot: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Rad51C detected in HEK293 lysate using NB 100-177. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School. Image from the standard format of this antibody.![Flow Cytometry: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923]](https://resources.rndsystems.com/images/products/Rad51C-Antibody-2H11-6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80923-img0003.jpg "Flow Cytometry: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923]")
Flow Cytometry: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923]
Flow Cytometry: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Intracellular flow cytometric staining of 1 x 10^6 HeLa cells using Rad51C antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.![Western Blot: Rad51C Antibody (2H11/6)Azide and BSA Free [NBP2-80923]](https://resources.rndsystems.com/images/products/Rad51C-Antibody-2H11-6-Azide-and-BSA-Free-Western-Blot-NBP2-80923-img0001.jpg "Western Blot: Rad51C Antibody (2H11/6)Azide and BSA Free [NBP2-80923]")
Western Blot: Rad51C Antibody (2H11/6)Azide and BSA Free [NBP2-80923]
Western Blot: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Analysis of RAD51c in 1) Hela WCE 2) HepG2 WCE 3) Cos-7 WCE 4) Hek293 WCE. Image from the standard format of this antibody.![Simple Western: Rad51C Antibody (2H11/6)Azide and BSA Free [NBP2-80923]](https://resources.rndsystems.com/images/products/Rad51C-Antibody-2H11-6-Azide-and-BSA-Free-Simple-Western-NBP2-80923-img0004.jpg "Simple Western: Rad51C Antibody (2H11/6)Azide and BSA Free [NBP2-80923]")
Simple Western: Rad51C Antibody (2H11/6)Azide and BSA Free [NBP2-80923]
Simple Western: Rad51C Antibody (2H11/6) - Azide and BSA Free [NBP2-80923] - Lane view shows a specific band for Rad51C in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.Applications for Rad51C Antibody (2H11/6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug per million cells
Immunohistochemistry-Paraffin
reported in scientific literature (PMID 23512992)
Simple Western
1:100
Western Blot
1:1000
Application Notes
In WB, a band can be seen at approx. 40 kDa. Preliminary feedback has been negative for Immunofluorescence on 4% PFA-fixed human cell lines (H1299 and MCF7).
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:100. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. This antibody is CyTOF ready.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Rad51C
Alternate Names
BROVCA3, DNA repair protein RAD51 homolog 3, FANCO, MGC104277, R51H3, RAD51 homolog C, RAD51 homolog C (S. cerevisiae), RAD51L2RAD51 (S. cerevisiae) homolog C, RAD51-like protein 2, yeast RAD51 homolog 3
Gene Symbol
RAD51C
Additional Rad51C Products
Product Documents for Rad51C Antibody (2H11/6) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Rad51C Antibody (2H11/6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Rad51C Antibody (2H11/6) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review Rad51C Antibody (2H11/6) - Azide and BSA Free and earn rewards!
Have you used Rad51C Antibody (2H11/6) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...