Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # RAP1 4C8/1

Format

BSA Free
View all Rap1A Primary Antibodies »

Product Specifications

Immunogen

Human RAP1A

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for RAP1A Antibody (RAP1 4C8/1) - BSA Free

Immunocytochemistry/ Immunofluorescence: RAP1A Antibody (RAP1 4C8/1) - BSA Free [NBP2-50269]

Immunocytochemistry/ Immunofluorescence: RAP1A Antibody (RAP1 4C8/1) - BSA Free [NBP2-50269]

Immunocytochemistry/Immunofluorescence: RAP1A Antibody (RAP1 4C8/1) [NBP2-50269] - Staining of MEFs with anti-RAP1 [RAP1 4C8/1] antibody.

Applications for RAP1A Antibody (RAP1 4C8/1) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): HeLa cell and mouse testis extracts. This antibody is Cytof ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.43 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rap1A

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Ras-related Protein 1A

Alternate Names

KREV-1, SMGP21

Gene Symbol

RAP1A

UniProt

A2ALS5 (Mouse)

Additional Rap1A Products

Product Documents for RAP1A Antibody (RAP1 4C8/1) - BSA Free

Certificate of Analysis

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Product Specific Notices for RAP1A Antibody (RAP1 4C8/1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAP1A Antibody (RAP1 4C8/1) - BSA Free

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Protocols

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FAQs

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