RAP80 Antibody

Novus Biologicals | Catalog # NBP1-45228

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RAP80 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-TRRQKEAKTKSDSG corresponding to internal region according to NP_057374.3.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RAP80 Antibody

Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-45228]

Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-45228]

Immunohistochemistry-Paraffin: RAP80 Antibody [NBP1-45228] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RAP80 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 ug/ml
Application Notes
WB: Preliminary experiments gave an approx. 60 kDa band in human heart and spleen lysates and in lysates of A431, HeLa and HepG2 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.7 kDa band according to NP_057374.3. The 60 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Testis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAP80

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. TRAP80 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. TRAP80 is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animals cells produce the protein, which stimulates the animals immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Long Name

Receptor Associated Protein 80

Alternate Names

RXRIP110, UIMC1, X2HRIP110

Entrez Gene IDs

51720 (Human); 20184 (Mouse); 290997 (Rat)

Gene Symbol

UIMC1

Additional RAP80 Products

Product Documents for RAP80 Antibody

Certificate of Analysis

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Product Specific Notices for RAP80 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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