RAR alpha/NR1B1 Antibody (2D2) - Azide and BSA Free

Novus Biologicals | Catalog # H00005914-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D2

Format

Azide and BSA Free
View all RAR alpha/NR1B1 Primary Antibodies »

Product Specifications

Immunogen

RARA (NP_000955, 315 a.a. ~ 424 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLS

Specificity

RARA (2D2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RAR alpha/NR1B1 Antibody (2D2) - Azide and BSA Free

Western Blot: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03]

Western Blot: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03]

Western Blot: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03] - Analysis of RARA expression in Hela S3 NE (Cat # L013V3).
Immunohistochemistry-Paraffin: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03]

Immunohistochemistry-Paraffin: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03]

Immunohistochemistry-Paraffin: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03] - Analysis of monoclonal antibody to RARA on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 1.2 ug/ml
ELISA: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03]

ELISA: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03]

ELISA: RAR alpha/NR1B1 Antibody (2D2) [H00005914-M03] - Detection limit for recombinant GST tagged RARA is approximately 1ng/ml as a capture antibody.

Applications for RAR alpha/NR1B1 Antibody (2D2) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAR alpha/NR1B1

Retinoid signaling is transduced by 2 families of nuclear receptors, retinoic acid receptor (RAR) and retinoid X receptor (RXR; see MIM 180245), which form RXR/RAR heterodimers. In the absence of ligand, DNA-bound RXR/RARA represses transcription by recruiting the corepressors NCOR1 (MIM 600849), SMRT (NCOR2; MIM 600848), and histone deacetylase (see MIM 601241). When ligand binds to the complex, it induces a conformational change allowing the recruitment of coactivators, histone acetyltransferases (see MIM 603053), and the basic transcription machinery. Translocations that always involve rearrangement of the RARA gene are a cardinal feature of acute promyelocytic leukemia (APL; MIM 612376). The most frequent translocation is t(15,17)(q21;q22), which fuses the RARA gene with the PML gene (MIM 102578) (Vitoux et al., 2007 [PubMed 17468032]).[supplied by OMIM]

Long Name

Retinoic Acid Receptor, alpha

Alternate Names

NR1B1, RARA

Entrez Gene IDs

5914 (Human)

Gene Symbol

RARA

OMIM

180240 (Human)

UniProt

NP_000955 (Human)

Additional RAR alpha/NR1B1 Products

Product Documents for RAR alpha/NR1B1 Antibody (2D2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RAR alpha/NR1B1 Antibody (2D2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RAR alpha/NR1B1 Antibody (2D2) - Azide and BSA Free

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Protocols

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FAQs

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