Rat Agrin Antibody

Catalog # Availability Size / Price Qty
AF550
AF550-SP
Acetylcholine Receptor Clustering Induced by Agrin and Neutralization by Rat Agrin Antibody.
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Product Details
Citations (2)
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Rat Agrin Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Agrin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant rat (rr) GDNF, rrIL-1 alpha, and rrL-Selectin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat Agrin
Ala1153-Pro1959 (Pro1788-Ser1798 del)
Accession # P25304
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Agrin (Catalog # 550-AG)
Immunohistochemistry
5-15 µg/mL
See below

Rat Agrin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Rat Agrin Biotinylated Antibody (Catalog # BAF550)

Standard: Recombinant Rat Agrin Protein (Catalog # 550-AG)

Neutralization
Measured by its ability to neutralize Agrin-induced acetylcholine receptor clustering on chick myotubes. The Neutralization Dose (ND50) is typically 0.001-0.004 µg/mL in the presence of 0.016 µg/mL Recombinant Rat Agrin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Acetylcholine Receptor Clustering Induced by Agrin and Neutralization by Rat Agrin Antibody. View Larger

Acetylcholine Receptor Clustering Induced by Agrin and Neutralization by Rat Agrin Antibody. Recombinant Rat Agrin (Catalog # 550-AG) induces acetylcholine receptor clustering on chick myotubes in a dose-dependent manner (orange line). Acetylcholine Receptor Clustering elicited by Recom­binant Rat Agrin (0.016 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Agrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF550). The ND50 is typically 0.001‑0.004 µg/mL.

Immunohistochemistry Agrin in Mouse Kidney. View Larger

Agrin in Mouse Kidney. Agrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Rat Agrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF550) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to basement membrane of epithelial cells in tubules and endothelial cells in glomeruli. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Agrin

Agrin, a heparan sulfate proteoglycan, is a component of the synaptic basal lamina which promotes acetylcholine receptor (AChR) clustering on cultured myotubes and in vivo. This AChR clustering activity has been shown to be mediated via a receptor complex that includes a receptor-like tyrosine kinase specific to the skeletal muscle termed muscle-specific kinase (MuSK), and an as of yet unidentified myotube-specific accessory component.

Agrin contains a number of distinct domains. The N-terminal half of the molecule, which is responsible for the tight interaction with the extracellular matrix, has nine follistatin-like repeats that share homology to Kazal-type protease inhibitor domains. The C-terminal half, which by itself is sufficient for the AChR clustering activity, has four EGF-like repeats and three laminin globular G domains. Agrin exists in several isoforms which are generated by alternative splicing at multiple splicing sites in the C-terminal half. Some of these isoforms are expressed specifically in the nervous system while other isoforms are expressed in both neural and nonneural tissues. Dramatic differences in AChR clustering activities have been observed between the different isoforms. The highest activity is found to be associated with isoforms found exclusively in neural tissues that contain the four amino acids K-S-R-K at position # 1643‑1646 and the eight amino acids E-L-T-N-E-I-P-A after Ser1779. Rat and chick Agrin share approximately 60% amino acid sequence identity. R&D Systems recombinant C-terminal half fragment of Agrin is a soluble secreted protein which has been found to have AChR clustering activity on chick myotubes.

References
  1. Kleiman, R.J. and L.F.Reichardt (1996) Cell 85:461.
  2. Glass, D. et al. (1996) Cell 85:513.
  3. Bowe, M. and J. Fallon (1995) Annu. Rev. Neurosci. 18:443.
Entrez Gene IDs
375790 (Human); 25592 (Rat)
Alternate Names
agrin proteoglycan; Agrin; AGRN

Product Datasheets

Citations for Rat Agrin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Agrin, a novel basement membrane component in human and rat liver, accumulates in cirrhosis and hepatocellular carcinoma.
    Authors: Tatrai P, Dudas J, Batmunkh E, Máthé M, Zalatnai A, Schaff Z, Ramadori G, Kovalszky I
    Lab. Invest., 2006;86(11):1149-60.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  2. Neurexins induce differentiation of GABA and glutamate postsynaptic specializations via neuroligins.
    Authors: Graf ER, Zhang X, Jin SX, Linhoff MW, Craig AM
    Cell, 2004;119(7):1013-26.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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