Rat CD19 Alexa Fluor® 594-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CD19

CD19 (also surface antigen B4 and Leu12) is a 95‑110 kDa member of the Immunoglobulin superfamily of molecules. It is expressed by B cells, and interacts with CD21 for the purpose of reducing the threshold of the antigen signal needed to with activate the BCR. CD19 ligation also promotes B cell:follicular dendritic cell (FDC) interaction and B cell proliferation in the FDC zone of the spleen. Mature rat CD19 is a 529 amino acid (aa) type I transmembrane glycoprotein (aa 19‑547). Based on mouse, it contains a 269 aa extracellular region (aa 19‑287) plus a 236 aa cytoplasmic domain. The extracellular region possesses two C2-type Ig-like domains (aa 20‑113 and 171‑271) and one utilized phosphorylation site at Ser225. The cytoplasmic domain contains five potential Tyr phosphorylation sites. There is one splice form that shows a two aa substitution after Gly489. Over aa 19‑287, rat CD19 shares 88% and 57% aa sequence identity with mouse and human CD19, respectively.

Product Datasheets

Related Research Areas

• Acute Lymphoblastic Leukemia
• B Cell Activation
• B Cell Development and Differentiation
• B Cell Markers
• Cancer Stem Cell Markers
• Conventional/Classical Dendritic Cells
• Inflammatory/Monocyte-derived Dendritic Cells
• Langerhans and Dermal Dendritic Cells
• Lymphoid Lineage Markers
• Multiple Myeloma
• Natural Killer T (NKT) Cells
• Plasmacytoid Dendritic Cells