Rat CD25/IL-2 R alpha PE-conjugated Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat IL-2 R alpha in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-2 R alpha, rmIL-2 R beta, rmCommon gamma chain, rmIL-15 R alpha, or recombinant human IL-2 R alpha is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 745520
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant rat IL-2 R alpha
    Glu22-Gln235
    Accession # P26897
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD25/IL-2 R alpha in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with APC-conjugated anti-rat CD4 antibody and either (A) Mouse Anti-Rat CD25/IL-2 R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB51561P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD25/IL-2 R alpha

IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I transmembrane (TM) glycoprotein that functionally belongs to a family of cytokine receptors that utilize the common gamma chain subunit ( gamma c) (1-3). IL-2 R alpha is expressed on activated T cells, and on regulatory T cells (Treg), mast cells, fibroblasts, NK cells, endthelial cells and pre-B cells (4-6). The rat IL-2 R alpha cDNA encodes a 267 amino acid (aa) precursor that includes a 21 aa signal peptide, a 214 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain (7). Within the ECD, rat IL-2 R alpha shares 58% and 81% aa sequence identity with human and mouse IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. By itself, IL-2 R alpha binds IL-2 with low affinity. It then associates with IL-2 R beta and subsequently to gamma c to generate a ternary high affinity IL-2 receptor complex. Notably, it would appear that IL-2 R alpha also plays a role in the formation of supramolecular membrane complexes that also include MHC-II molecules and additional gamma c -related alpha -chains (8).  A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2 (9, 10). Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders (11). IL-2 R alpha is required for activation induced cell death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (12, 13). IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis (12-15).

  • References:
    1. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
    2. Kovanen, P.E. and W.J. Leonard (2004) Immunol. Rev. 202:67.
    3. Bluestone, J.A. and Q. Tang (2005) Curr. Opin. Immunol. 17:638.
    4. Alvarez-Twose, I. et al. (2012) Int. J. Lab. Hematol. 116:117. 
    5. Letourneau, S. et al. (2009) J. Allergy Clin. Immunol. 123:758.  
    6. Malek, T.R. and I. Castro (2010) Immunity 33:153. 
    7. Page, T.H. and M.J. Dallman (1991) Eur. J. Immunol. 21:2133.
    8. Nizsaloczki, E. et al. (2014) Chempsychem. 15:3969.  
    9. Wagner, D.K. et al. (1986) J. Immunol. 137:592.
    10. Schulz, O. et al. (1998) J. Exp. Med. 187:271.
    11. Witkowska, A.M. (2005) Mediators Inflamm. 2005:121.
    12. Willerford, D.M. et al. (1995) Immunity 3:521.
    13. Van Parijs, L. et al. (1997) J. Immunol. 158:3738.
    14. Almeida, A.R.M. et al. (2002) J. Immunol. 169:4850.
    15. Bayer, A.L. et al. (2013) Immunol. Res. 57:197.
  • Long Name:
    Interleukin 2 Receptor alpha
  • Entrez Gene IDs:
    3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine)
  • Alternate Names:
    CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL2R alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2Ra; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
Related Research Areas

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