Rat CD44 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects rat CD44 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human, mouse, or porcine CD44 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 740017
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat CD44
    Gln22-Thr223 (predicted)
    Accession # P26051
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD44 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Rat CD4 APC‑conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat CD44 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB6577G) or (B) Mouse IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD44

CD44 is a ubiquitously expressed protein that is a major receptor for hyaluronan and exerts control over cell growth and migration (1-5). Mouse CD44 has an extracellular domain (ECD) that contains a 100 amino acid (aa) hyaluronan-binding disulfide-stabilized link region, plus a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1‑5). The standard or hematopoietic form, CD44H, does not include the variable segments (1‑5). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (2, 4). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1, 2). Within the N‑terminal invariant portion of the ECD (aa 23-222), rat CD44 shares 91% and 75% aa sequence identity with corresponding mouse and human CD44, respectively. The many reported functions of CD44 fall within three categories (1, 2). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 is a co-receptor that modifies activity of receptors including MET and the ErbB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases or ADAM proteases to produce soluble ectodomains (4, 6, 7). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta ‑like transmembrane portion and a cytoplasmic signaling molecule that affects gene expression (8, 9). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 2, 6).

  • References:
    1. Pure, E. and R.K. Assoian (2009) Cell. Signal. 21:651.
    2. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
    3. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
    4. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
    5. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
    6. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
    7. Nakamura, H. et al. (2004) Cancer Res. 64:876.
    8. Murakami, D. et al. (2003) Oncogene 22:1511.
    9. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
  • Entrez Gene IDs:
    960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
  • Alternate Names:
    CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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