Rat CNTF Antibody Summary
Accession # P20294
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CNTF in Rat Spinal Cord. CNTF was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glial cells in the white matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neuron Survival in Response to CNTF and Neutralization by Rat CNTF Antibody. Recombinant Rat CNTF (Catalog # 557-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Rat CNTF (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA). The ND50is typically 7-15 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.
Citation for Rat CNTF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Ciliary neurotrophic factor mediates dopamine D2 receptor-induced CNS neurogenesis in adult mice.
Authors: Yang P, Arnold SA, Habas A, Hetman M, Hagg T
J. Neurosci., 2008;28(9):2231-41.
Sample Types: In Vivo
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