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Rat EphB1 Antibody

Catalog #: MAB5421 Datasheet / COA / SDS

Discontinued Product

MAB5421 has been discontinued.
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Rat EphB1 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat EphB1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse EphA1, A3, A4, A6, A7, A8, B2, B3 or recombinant human EphA1, A2, A5, B4, or B6 is observed.
Source
Monoclonal Mouse IgG1 Clone # 88512
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat EphB1
Met18-Gln538
Accession # P09759
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
NCI‑H345 human small cell lung carcinoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
Immersion fixed NCI-H345 human small cell lung carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB1

EphB1, also known as Elk, Cek6, Net, and Hek6, is a type I transmembrane receptor tyrosine kinase with two extracellular fibronectin type III domains. EphB1 binds Ephrin B1, B2, and B3 and plays an important role in nervous system development and axonal migration. Within the extracellular domain, rat EphB1 shares 99% amino acid sequence identity with human and mouse EphB1.

Long Name
Eph Receptor B1
Entrez Gene IDs
2047 (Human); 270190 (Mouse); 24338 (Rat)
Alternate Names
Cek6; EC 2.7.10; EC 2.7.10.1; EK6; Elk; EPH receptor B1; eph tyrosine kinase 2; EphB1; EPH-like kinase 6; ephrin type-B receptor 1; EPHT2FLJ37986; Hek6; Net; NETHek6; soluble EPHB1 variant 1; Tyrosine-protein kinase receptor EPH-2

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