Rat GFR alpha -1/GDNF R alpha -1 Biotinylated Antibody

(2 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat GFR alpha ‑1/GDNF R alpha ‑1 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat GFR alpha ‑1/GDNF R alpha ‑1
    Asp25-Leu445
    Accession # Q62997
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 560-GR)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
GDNF and GFR alpha ‑1 in Rat Dorsal Root Ganglia. GDNF and GFR alpha ‑1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Goat AntiRat Anti-GFR alpha ‑1 Biotinylated Antigen Affinity-purified Poly­clonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFR alpha-1/GDNF R alpha-1
Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.
  • References:
    1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
    2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
    3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  • Long Name:
    Glial Cell line-derived Neurotrophic Factor Receptor alpha 1
  • Entrez Gene IDs:
    2674 (Human); 25454 (Rat)
  • Alternate Names:
    DKFZp313E1012; DKFZp686J0156; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF R alpha-1; GDNF receptor alpha-1; GDNFRAGFR-ALPHA-1; GDNFR-alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; GFRA1; GFRa-1; GFR-alpha-1; Glial cell line-derived neurotrophic factor receptor alpha; GPI-linked anchor protein; PI-linked cell-surface accessory protein; RET ligand 1; RET1L; RETL1FLJ10561; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR1FLJ31546
Related Research Areas
Citations:

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2 Citations: Showing 1 - 2
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Sample Type
  1. GDNF availability determines enteric neuron number by controlling precursor proliferation.
    Authors: Gianino S, Grider JR, Cresswell J, Enomoto H, Heuckeroth RO
    Development, 2003;130(10):2187-98.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. TGFbeta induces GDNF responsiveness in neurons by recruitment of GFRalpha1 to the plasma membrane.
    Authors: Peterziel H, Unsicker K, Krieglstein K
    J. Cell Biol., 2002;159(1):157-67.
    Species: Chicken
    Sample Type: Cell Lysates
    Application: WB
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