Human/Rat GDNF Antibody

Catalog # Availability Size / Price Qty
AF-212-NA
AF-212-SP
GDNF and GFR alpha ‑1 in Rat Dorsal Root Ganglia.
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Product Details
Citations (28)
FAQs
Supplemental Products
Reviews (2)

Human/Rat GDNF Antibody Summary

Species Reactivity
Human, Rat
Specificity
Detects human and rat GDNF in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived and Mouse myeloma cell line NS0-derived recombinant human GDNF
Arg109-Ile211
Accession # P39905
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human GDNF (Catalog # 212-GD)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize GDNF-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND50) is typically 0.25-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry GDNF and GFR alpha ‑1 in Rat Dorsal Root Ganglia. View Larger

GDNF and GFR alpha ‑1 in Rat Dorsal Root Ganglia. GDNF and GFR alpha ‑1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Biotinylated Goat Anti-Rat GFR alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Neutralization Proliferation Induced by GDNF and Neutralization by Goat GDNF Antibody. View Larger

Proliferation Induced by GDNF and Neutralization by Goat GDNF Antibody. Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH‑SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-212-NA). The ND50 is typically 0.25-1 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Long Name
Glial Cell line-derived Growth Factor
Entrez Gene IDs
2668 (Human); 25453 (Rat)
Alternate Names
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; hGDNF; HSCR3

Product Datasheets

Citations for Human/Rat GDNF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
    Authors: R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Applications: Affinity Assay
  2. Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane
    Authors: Y Dong, A Kanda, K Noda, W Saito, S Ishida
    Sci Rep, 2017;7(0):44266.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC paraffin embedded
  3. The Noble Gas Xenon Provides Protection and Trophic Stimulation to Midbrain Dopamine Neurons
    Authors: J Lavaur, D Le Nogue, M Lemaire, J Pype, G Farjot, EC Hirsch, PP Michel
    J. Neurochem., 2017;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neut
  4. Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury
    Authors: K Nagashima, T Miwa, H Soumiya, D Ushiro, T Takeda-Kaw, N Tamaoki, S Ishiguro, Y Sato, K Miyamoto, T Ohno, M Osawa, T Kunisada, T Shibata, KI Tezuka, S Furukawa, H Fukumitsu
    Sci Rep, 2017;7(1):13500.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Systemic injection of AAV9-GDNF provides modest functional improvements in the SOD1(G93A) ALS rat but has adverse side effects
    Authors: GM Thomsen, M Alkaslasi, JP Vit, G Lawless, M Godoy, G Gowing, O Shelest, CN Svendsen
    Gene Ther, 2017;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's disease
    Authors: SR Stott, S Hayat, T Carnwath, S Garas, JP Sleeman, RA Barker
    PLoS ONE, 2017;12(2):e0171748.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair.
    Authors: Wang T, Bruggeman K, Sheean R, Turner B, Nisbet D, Parish C
    J Biol Chem, 2014;289(21):15044-51.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: ELISA detection
  8. beta-Catenin overexpression in the metanephric mesenchyme leads to renal dysplasia genesis via cell-autonomous and non-cell-autonomous mechanisms.
    Authors: Sarin S, Boivin F, Li A, Lim J, Svajger B, Rosenblum N, Bridgewater D
    Am J Pathol, 2014;184(5):1395-410.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  9. Clearance and toxicity of recombinant methionyl human glial cell line-derived neurotrophic factor (r-metHu GDNF) following acute convection-enhanced delivery into the striatum.
    Authors: Taylor H, Barua N, Bienemann A, Wyatt M, Castrique E, Foster R, Luz M, Fibiger C, Mohr E, Gill S
    PLoS ONE, 2013;8(3):e56186.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  10. Functional neuroprotection and efficient regulation of GDNF using destabilizing domains in a rodent model of Parkinson's disease.
    Authors: Quintino L, Manfre G, Wettergren E, Namislo A, Isaksson C, Lundberg C
    Mol Ther, 2013;21(12):2169-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  11. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012;7(6):e39036.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  12. Interventional MRI-guided putaminal delivery of AAV2-GDNF for a planned clinical trial in Parkinson's disease.
    Authors: Richardson RM, Kells AP, Rosenbluth KH, Salegio EA, Fiandaca MS, Larson PS, Starr PA, Martin AJ, Lonser RR, Federoff HJ, Forsayeth JR, Bankiewicz KS
    Mol. Ther., 2011;19(6):1048-57.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  13. Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain.
    Authors: Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS
    Neuropharmacology, 2010;58(7):1114-21.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  14. Efficient gene therapy-based method for the delivery of therapeutics to primate cortex.
    Authors: Kells AP, Hadaczek P, Yin D, Bringas J, Varenika V, Forsayeth J, Bankiewicz KS
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(7):2407-11.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC
  15. Response of aged parkinsonian monkeys to in vivo gene transfer of GDNF.
    Authors: Emborg ME, Moirano J, Raschke J, Bondarenko V, Zufferey R, Peng S, Ebert AD, Joers V, Roitberg B, Holden JE, Koprich J, Lipton J, Kordower JH, Aebischer P
    Neurobiol. Dis., 2009;36(2):303-11.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  16. Direct muscle delivery of GDNF with human mesenchymal stem cells improves motor neuron survival and function in a rat model of familial ALS.
    Authors: Suzuki M, McHugh J, Tork C, Shelley B, Hayes A, Bellantuono I, Aebischer P, Svendsen CN
    Mol. Ther., 2008;16(12):2002-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  17. Identification of neuregulin as a factor required for formation of aligned spermatogonia.
    Authors: Hamra FK, Chapman KM, Nguyen D, Garbers DL
    J. Biol. Chem., 2007;282(1):721-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neut
  18. SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
    Authors: Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP
    Development, 2007;134(18):3327-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  19. Human neural stem cell grafts ameliorate motor neuron disease in SOD-1 transgenic rats.
    Authors: Xu L, Yan J, Chen D, Welsh AM, Hazel T, Johe K, Hatfield G, Koliatsos VE
    Transplantation, 2006;82(7):865-75.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  20. Glial cell-line-derived neurotropic factor and its receptors are expressed by germinal and somatic cells of the rat testis.
    Authors: Fouchecourt S, Godet M, Sabido O, Durand P
    J. Endocrinol., 2006;190(1):59-71.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: WB
  21. Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
    Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O
    Stroke, 2006;37(9):2361-7.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  22. Anatomical basis of glial cell line-derived neurotrophic factor expression in the striatum and related basal ganglia during postnatal development of the rat.
    Authors: Oo TF, Ries V, Cho J, Kholodilov N, Burke RE
    J. Comp. Neurol., 2005;484(1):57-67.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  23. Rapid axoglial signaling mediated by neuregulin and neurotrophic factors.
    Authors: Esper RM, Loeb JA
    J. Neurosci., 2004;24(27):6218-27.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Neut
  24. Therapeutic immunization protects dopaminergic neurons in a mouse model of Parkinson's disease.
    Authors: Benner EJ, Mosley RL, Destache CJ, Lewis TB, Jackson-Lewis V, Gorantla S, Nemachek C, Green SR, Przedborski S, Gendelman HE
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(25):9435-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Recombinant adeno-associated viral vector (rAAV) delivery of GDNF provides protection against 6-OHDA lesion in the common marmoset monkey (Callithrix jacchus).
    Authors: Eslamboli A, Cummings RM, Ridley RM, Baker HF, Muzyczka N, Burger C, Mandel RJ, Kirik D, Annett LE
    Exp. Neurol., 2003;184(1):536-48.
    Species: Primate - Callitrix jacchus (Common Marmoset)
    Sample Types: Whole Tissue
    Applications: IHC
  26. Response of motoneurons to neonatal sciatic nerve axotomy in Bax-knockout mice.
    Authors: Sun W, Oppenheim RW
    Mol. Cell. Neurosci., 2003;24(4):875-86.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo
  27. Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate.
    Authors: Honma M, Namikawa K, Mansur K, Iwata T, Mori N, Iizuka H, Kiyama H
    J. Neurochem., 2002;82(4):961-75.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  28. Immunohistochemical localization of GDNF in the human hippocampal formation from prenatal life to adulthood.
    Authors: Pina Serra M, Quartu M, Ambu R, Follesa P, Del Fiacco M
    Brain Res., 2002;928(1):138-46.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: WB

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Reviews for Human/Rat GDNF Antibody

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Human/Rat GDNF Antibody
By Anonymous on 08/29/2017
Application: ELISA Sample Tested: Serum Species: Guinea Pig

anti h/rGDNF was used to make positive control for competitive elisa to determine GDNF in Guinea pig serum. Works perfect.


Human/Rat GDNF Antibody
By Anonymous on 07/19/2017
Application: ELISA Sample Tested: Porcine Serum Species: Rat

Used for competitive elisa using serum