Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat, Avian - Chicken, Primate - Callithrix jacchus (Common Marmoset), Primate - Macaca mulatta (Rhesus Macaque), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Affinity Assay, ELISA Development (Detection)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GDNF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived and Mouse myeloma cell line NS0-derived recombinant human GDNF
Arg109-Ile211
Accession # P39905

Specificity

Detects human and rat GDNF in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human/Rat GDNF Antibody

GDNF and GFRa-1 antibody in Rat Dorsal Root Ganglia by Immunohistochemistry (IHC-Fr).

GDNF and GFR alpha ‑1 in Rat Dorsal Root Ganglia.

GDNF and GFRa-1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Biotinylated Goat Anti-Rat GFRa-1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Proliferation Induced by GDNF and Neutralization by Goat GDNF Antibody.

Proliferation Induced by GDNF and Neutralization by Goat GDNF Antibody.

Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH-SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFRa-1/GDNF Ra-1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-212-NA). The ND50 is typically 0.25-1 µg/mL.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF)-induced claudin-5 & VE-cadherin expression in hCMEC/D3 by activating the PI3K/AKT & MAPK/ERK signaling.(A) Effects of 3 μM LY294002 (LY) on levels of claudin-5, VE-cadherin, & p-AKT/AKT in hCMEC/D3 stimulated by 200 pg/ml GDNF. (B) Effects of 2 μM U0126 (U0) on levels of claudin-5, VE-cadherin, & p-ERK/ERK in hCMEC/D3 stimulated by 200 pg/ml GDNF. (C) Effects of 5 μM SP600125 (SP) on levels of claudin-5, VE-cadherin, & p-JNK/JNK in hCMEC/D3 stimulated by 200 pg/ml GDNF. (D) Effects of 2 μM SB203580 (SB) on levels of claudin-5, VE-cadherin, & p-p38/p38 in hCMEC/D3 stimulated by 200 pg/ml GDNF. (E) Effects of anti-GDNF antibody on GDNF-induced p-AKT/AKT & p-ERK/ERK ratios. (F) Effects of 3 μM LY on levels of claudin-5, VE-cadherin, & p-AKT/AKT in hCMEC/D3 stimulated by US-CM. (G) Effects of 2 μM U0 on levels of claudin-5, VE-cadherin, & p-ERK/ERK in hCMEC/D3 stimulated by US-CM. (H) Effects of 5 μM SP on levels of claudin-5, VE-cadherin, & p-JNK/JNK in hCMEC/D3 stimulated by US-CM. (I) Effects of 2 μM SB on levels of claudin-5, VE-cadherin, & p-p38/p38 in hCMEC/D3 stimulated by US-CM. (J) Effects of anti-GDNF antibody on US-CM-induced p-AKT/AKT & p-ERK/ERK ratios. The above data are shown as the mean ± SEM. Four biological replicates per group. One technical replicate per biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test or Welch’s ANOVA test. Figure 3—source data 1.The WB raw images in Figure 3.Figure 3—source data 2.The labeled WB images in Figure 3.Figure 3—source data 3.Excel file containing summary data & data analysis of Figure 3.The WB raw images in Figure 3.The labeled WB images in Figure 3.Excel file containing summary data & data analysis of Figure 3. Image collected & cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF) induced the claudin-5 expression in hCMEC/D3 cells by activating the PI3K/AKT/FOXO1 pathway. Effects of US-CM and GDNF on the phosphorylated FOXO1 (p-FOXO1)/FOXO1 ratio, total FOXO1 expression (A). Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF)-induced VE-cadherin expression in hCMEC/D3 cells by activating the PI3K/AKT/ETS1 and MAPK/ERK/ETS1 pathways. Effects of US-CM and GDNF on total (A) and nuclear (B) ETS1 expression. Effects of LY and U0 on 200 pg/ml GDNF-induced total (C) and nuclear (D) ETS1 expression. Expression levels of total (E) and the nuclear ETS1 (F) in hCMEC/D3 cells after knocking down ETS1 with siRNA (siETS1). (G) Effects of GDNF and siETS1 on the expression of VE-cadherin and claudin-5. The above data are shown as the mean ± SEM. Four biological replicates per group. One technical replicate for each biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test. Figure 5—source data 1.The western blot raw images in Figure 5.Figure 5—source data 2.The labeled western blot images in Figure 5.Figure 5—source data 3.Excel file containing summary data and data analysis of Figure 5.The western blot raw images in Figure 5.The labeled western blot images in Figure 5.Excel file containing summary data and data analysis of Figure 5. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF)-induced claudin-5 & VE-cadherin expression in hCMEC/D3 by activating the PI3K/AKT & MAPK/ERK signaling.(A) Effects of 3 μM LY294002 (LY) on levels of claudin-5, VE-cadherin, & p-AKT/AKT in hCMEC/D3 stimulated by 200 pg/ml GDNF. (B) Effects of 2 μM U0126 (U0) on levels of claudin-5, VE-cadherin, & p-ERK/ERK in hCMEC/D3 stimulated by 200 pg/ml GDNF. (C) Effects of 5 μM SP600125 (SP) on levels of claudin-5, VE-cadherin, & p-JNK/JNK in hCMEC/D3 stimulated by 200 pg/ml GDNF. (D) Effects of 2 μM SB203580 (SB) on levels of claudin-5, VE-cadherin, & p-p38/p38 in hCMEC/D3 stimulated by 200 pg/ml GDNF. (E) Effects of anti-GDNF antibody on GDNF-induced p-AKT/AKT & p-ERK/ERK ratios. (F) Effects of 3 μM LY on levels of claudin-5, VE-cadherin, & p-AKT/AKT in hCMEC/D3 stimulated by US-CM. (G) Effects of 2 μM U0 on levels of claudin-5, VE-cadherin, & p-ERK/ERK in hCMEC/D3 stimulated by US-CM. (H) Effects of 5 μM SP on levels of claudin-5, VE-cadherin, & p-JNK/JNK in hCMEC/D3 stimulated by US-CM. (I) Effects of 2 μM SB on levels of claudin-5, VE-cadherin, & p-p38/p38 in hCMEC/D3 stimulated by US-CM. (J) Effects of anti-GDNF antibody on US-CM-induced p-AKT/AKT & p-ERK/ERK ratios. The above data are shown as the mean ± SEM. Four biological replicates per group. One technical replicate per biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test or Welch’s ANOVA test. Figure 3—source data 1.The WB raw images in Figure 3.Figure 3—source data 2.The labeled WB images in Figure 3.Figure 3—source data 3.Excel file containing summary data & data analysis of Figure 3.The WB raw images in Figure 3.The labeled WB images in Figure 3.Excel file containing summary data & data analysis of Figure 3. Image collected & cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

The deficiency of brain glial cell line-derived neurotrophic factor (GDNF) in mice increased the permeability of blood–brain barrier (BBB) and reduced claudin-5 and VE-cadherin expression in mice brains.(A) Experimental configuration of AAV-GFP (shNC) or AAV-shGdnf (shGdnf) intracerebroventricular injection. (B) Effects of brain-specific Gdnf silencing on the expression levels of GDNF, claudin-5, and VE-cadherin in the brains. Effects of brain-specific Gdnf silencing on NaF levels in plasma (C), brain (D), and the ratio of brain to plasma (E). Effects of brain-specific Gdnf silencing on FITC-Dex levels in plasma (F), brain (G), and the ratio of brain to plasma (H). The expression ratios of p-AKT/AKT (I), p-ERK/ERK (J), and p-FOXO1/FOXO1 (K) in the brains of Gdnf silencing mice. (L) The expression level of ETS1 in the brains of Gdnf silencing mice. The above data are shown as the mean ± SEM. Six biological replicates per group. One technical replicate for each biological replicate. *p < 0.05; **p < 0.01 by unpaired t-test, unpaired t-test with Welch’s correction, or Mann–Whitney test. Figure 6—source data 1.The western blot raw images in Figure 6.Figure 6—source data 2.The labeled western blot images in Figure 6.Figure 6—source data 3.Excel file containing summary data and data analysis of Figure 6.The western blot raw images in Figure 6.The labeled western blot images in Figure 6.Excel file containing summary data and data analysis of Figure 6. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF) induced the claudin-5 expression in hCMEC/D3 cells by activating the PI3K/AKT/FOXO1 pathway. (G) Effects of LY on the claudin-5 expression upregulated by siFOXO1. The above data are shown as the mean± SEM. Four biological replicates per group. One technical replicate for each biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test, Welch’s ANOVA test, or Kruskal–Wallis test. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF) induced the claudin-5 expression in hCMEC/D3 cells by activating the PI3K/AKT/FOXO1 pathway. The expression levels of claudin-5, and VE-cadherin (D) in hCMEC/D3 cells transfected with FOXO1 siRNA (siFOXO1). NC: negative control. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Neurons and astrocytes upregulated claudin-5 and VE-cadherin expression in hCMEC/D3 cells due to glial cell line-derived neurotrophic factor (GDNF) secretion. Effects of 3 μM RET tyrosine kinase inhibitor SSP-86 (SPP), and 5 μM Src family kinases inhibitor PP2 on the upregulation of claudin-5 and VE-cadherin induced by 200 pg/mL GDNF (K). Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Neurons and astrocytes upregulated claudin-5 and VE-cadherin expression in hCMEC/D3 cells due to glial cell line-derived neurotrophic factor (GDNF) secretion. Effects of TGF-beta (G) on the expression of claudin-5 and VE-cadherin. The dosages have been marked in the figure. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF)-induced claudin-5 & VE-cadherin expression in hCMEC/D3 by activating the PI3K/AKT & MAPK/ERK signaling.(A) Effects of 3 μM LY294002 (LY) on levels of claudin-5, VE-cadherin, & p-AKT/AKT in hCMEC/D3 stimulated by 200 pg/ml GDNF. (B) Effects of 2 μM U0126 (U0) on levels of claudin-5, VE-cadherin, & p-ERK/ERK in hCMEC/D3 stimulated by 200 pg/ml GDNF. (C) Effects of 5 μM SP600125 (SP) on levels of claudin-5, VE-cadherin, & p-JNK/JNK in hCMEC/D3 stimulated by 200 pg/ml GDNF. (D) Effects of 2 μM SB203580 (SB) on levels of claudin-5, VE-cadherin, & p-p38/p38 in hCMEC/D3 stimulated by 200 pg/ml GDNF. (E) Effects of anti-GDNF antibody on GDNF-induced p-AKT/AKT & p-ERK/ERK ratios. (F) Effects of 3 μM LY on levels of claudin-5, VE-cadherin, & p-AKT/AKT in hCMEC/D3 stimulated by US-CM. (G) Effects of 2 μM U0 on levels of claudin-5, VE-cadherin, & p-ERK/ERK in hCMEC/D3 stimulated by US-CM. (H) Effects of 5 μM SP on levels of claudin-5, VE-cadherin, & p-JNK/JNK in hCMEC/D3 stimulated by US-CM. (I) Effects of 2 μM SB on levels of claudin-5, VE-cadherin, & p-p38/p38 in hCMEC/D3 stimulated by US-CM. (J) Effects of anti-GDNF antibody on US-CM-induced p-AKT/AKT & p-ERK/ERK ratios. The above data are shown as the mean ± SEM. Four biological replicates per group. One technical replicate per biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test or Welch’s ANOVA test. Figure 3—source data 1.The WB raw images in Figure 3.Figure 3—source data 2.The labeled WB images in Figure 3.Figure 3—source data 3.Excel file containing summary data & data analysis of Figure 3.The WB raw images in Figure 3.The labeled WB images in Figure 3.Excel file containing summary data & data analysis of Figure 3. Image collected & cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

The contribution of VE-cadherin on the glial cell line-derived neurotrophic factor (GDNF)-induced claudin-5 expression. Effects of the VE-cadherin siRNA (siVE-Cad) on mRNA expression of VE-cadherin (A) and claudin-5 (B). Effects of siVE-Cad and GDNF on claudin-5 and VE-cadherin protein expression (C). NC: negative control plasmids. The above data are shown as the mean ± SEM. Four biological replicates per group. Two technical replicates for A and B and one technical replicate for C. Statistical significance was determined using unpaired t-test or one-way ANOVA test followed by Fisher’s LSD test. Figure 4—figure supplement 1—source data 1.The western blot raw images in Figure 4—figure supplement 1.Figure 4—figure supplement 1—source data 2.The labeled western blot images in Figure 4—figure supplement 1.Figure 4—figure supplement 1—source data 3.Excel file containing summary data and data analysis of Figure 4—figure supplement 1.The western blot raw images in Figure 4—figure supplement 1.The labeled western blot images in Figure 4—figure supplement 1.Excel file containing summary data and data analysis of Figure 4—figure supplement 1. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF)-induced claudin-5 & VE-cadherin expression in hCMEC/D3 by activating the PI3K/AKT & MAPK/ERK signaling.(A) Effects of 3 μM LY294002 (LY) on levels of claudin-5, VE-cadherin, & p-AKT/AKT in hCMEC/D3 stimulated by 200 pg/ml GDNF. (B) Effects of 2 μM U0126 (U0) on levels of claudin-5, VE-cadherin, & p-ERK/ERK in hCMEC/D3 stimulated by 200 pg/ml GDNF. (C) Effects of 5 μM SP600125 (SP) on levels of claudin-5, VE-cadherin, & p-JNK/JNK in hCMEC/D3 stimulated by 200 pg/ml GDNF. (D) Effects of 2 μM SB203580 (SB) on levels of claudin-5, VE-cadherin, & p-p38/p38 in hCMEC/D3 stimulated by 200 pg/ml GDNF. (E) Effects of anti-GDNF antibody on GDNF-induced p-AKT/AKT & p-ERK/ERK ratios. (F) Effects of 3 μM LY on levels of claudin-5, VE-cadherin, & p-AKT/AKT in hCMEC/D3 stimulated by US-CM. (G) Effects of 2 μM U0 on levels of claudin-5, VE-cadherin, & p-ERK/ERK in hCMEC/D3 stimulated by US-CM. (H) Effects of 5 μM SP on levels of claudin-5, VE-cadherin, & p-JNK/JNK in hCMEC/D3 stimulated by US-CM. (I) Effects of 2 μM SB on levels of claudin-5, VE-cadherin, & p-p38/p38 in hCMEC/D3 stimulated by US-CM. (J) Effects of anti-GDNF antibody on US-CM-induced p-AKT/AKT & p-ERK/ERK ratios. The above data are shown as the mean ± SEM. Four biological replicates per group. One technical replicate per biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test or Welch’s ANOVA test. Figure 3—source data 1.The WB raw images in Figure 3.Figure 3—source data 2.The labeled WB images in Figure 3.Figure 3—source data 3.Excel file containing summary data & data analysis of Figure 3.The WB raw images in Figure 3.The labeled WB images in Figure 3.Excel file containing summary data & data analysis of Figure 3. Image collected & cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF)-induced VE-cadherin expression in hCMEC/D3 cells by activating the PI3K/AKT/ETS1 and MAPK/ERK/ETS1 pathways. Effects of US-CM and GDNF on total (A) and nuclear (B) ETS1 expression. Effects of LY and U0 on 200 pg/ml GDNF-induced total (C) and nuclear (D) ETS1 expression. Expression levels of total (E) and the nuclear ETS1 (F) in hCMEC/D3 cells after knocking down ETS1 with siRNA (siETS1). (G) Effects of GDNF and siETS1 on the expression of VE-cadherin and claudin-5. The above data are shown as the mean ± SEM. Four biological replicates per group. One technical replicate for each biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test. Figure 5—source data 1.The western blot raw images in Figure 5.Figure 5—source data 2.The labeled western blot images in Figure 5.Figure 5—source data 3.Excel file containing summary data and data analysis of Figure 5.The western blot raw images in Figure 5.The labeled western blot images in Figure 5.Excel file containing summary data and data analysis of Figure 5. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF)-induced VE-cadherin expression in hCMEC/D3 cells by activating the PI3K/AKT/ETS1 and MAPK/ERK/ETS1 pathways. Effects of US-CM and GDNF on total (A) and nuclear (B) ETS1 expression. Effects of LY and U0 on 200 pg/ml GDNF-induced total (C) and nuclear (D) ETS1 expression. Expression levels of total (E) and the nuclear ETS1 (F) in hCMEC/D3 cells after knocking down ETS1 with siRNA (siETS1). (G) Effects of GDNF and siETS1 on the expression of VE-cadherin and claudin-5. The above data are shown as the mean ± SEM. Four biological replicates per group. One technical replicate for each biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test. Figure 5—source data 1.The western blot raw images in Figure 5.Figure 5—source data 2.The labeled western blot images in Figure 5.Figure 5—source data 3.Excel file containing summary data and data analysis of Figure 5.The western blot raw images in Figure 5.The labeled western blot images in Figure 5.Excel file containing summary data and data analysis of Figure 5. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Neurons and astrocytes upregulated claudin-5 and VE-cadherin expression in hCMEC/D3 cells due to glial cell line-derived neurotrophic factor (GDNF) secretion. Effects of GDNF (D) on the expression of claudin-5 and VE-cadherin. The dosages have been marked in the figure. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF) induced the claudin-5 expression in hCMEC/D3 cells by activating the PI3K/AKT/FOXO1 pathway. (E) Effects of FOXO1 overexpression (FOXO1-OE) and GDNF on the expression levels of claudin-5, total FOXO1, and nuclear FOXO1. FOXO1-NC: negative control plasmids. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF)-induced claudin-5 & VE-cadherin expression in hCMEC/D3 by activating the PI3K/AKT & MAPK/ERK signaling.(A) Effects of 3 μM LY294002 (LY) on levels of claudin-5, VE-cadherin, & p-AKT/AKT in hCMEC/D3 stimulated by 200 pg/ml GDNF. (B) Effects of 2 μM U0126 (U0) on levels of claudin-5, VE-cadherin, & p-ERK/ERK in hCMEC/D3 stimulated by 200 pg/ml GDNF. (C) Effects of 5 μM SP600125 (SP) on levels of claudin-5, VE-cadherin, & p-JNK/JNK in hCMEC/D3 stimulated by 200 pg/ml GDNF. (D) Effects of 2 μM SB203580 (SB) on levels of claudin-5, VE-cadherin, & p-p38/p38 in hCMEC/D3 stimulated by 200 pg/ml GDNF. (E) Effects of anti-GDNF antibody on GDNF-induced p-AKT/AKT & p-ERK/ERK ratios. (F) Effects of 3 μM LY on levels of claudin-5, VE-cadherin, & p-AKT/AKT in hCMEC/D3 stimulated by US-CM. (G) Effects of 2 μM U0 on levels of claudin-5, VE-cadherin, & p-ERK/ERK in hCMEC/D3 stimulated by US-CM. (H) Effects of 5 μM SP on levels of claudin-5, VE-cadherin, & p-JNK/JNK in hCMEC/D3 stimulated by US-CM. (I) Effects of 2 μM SB on levels of claudin-5, VE-cadherin, & p-p38/p38 in hCMEC/D3 stimulated by US-CM. (J) Effects of anti-GDNF antibody on US-CM-induced p-AKT/AKT & p-ERK/ERK ratios. The above data are shown as the mean ± SEM. Four biological replicates per group. One technical replicate per biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test or Welch’s ANOVA test. Figure 3—source data 1.The WB raw images in Figure 3.Figure 3—source data 2.The labeled WB images in Figure 3.Figure 3—source data 3.Excel file containing summary data & data analysis of Figure 3.The WB raw images in Figure 3.The labeled WB images in Figure 3.Excel file containing summary data & data analysis of Figure 3. Image collected & cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF) induced the claudin-5 expression in hCMEC/D3 cells by activating the PI3K/AKT/FOXO1 pathway. The expression levels of total and nuclear FOXO1 (C) in hCMEC/D3 cells transfected with FOXO1 siRNA (siFOXO1). NC: negative control. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF)-induced VE-cadherin expression in hCMEC/D3 cells by activating the PI3K/AKT/ETS1 and MAPK/ERK/ETS1 pathways. Effects of US-CM and GDNF on total (A) and nuclear (B) ETS1 expression. Effects of LY and U0 on 200 pg/ml GDNF-induced total (C) and nuclear (D) ETS1 expression. Expression levels of total (E) and the nuclear ETS1 (F) in hCMEC/D3 cells after knocking down ETS1 with siRNA (siETS1). (G) Effects of GDNF and siETS1 on the expression of VE-cadherin and claudin-5. The above data are shown as the mean ± SEM. Four biological replicates per group. One technical replicate for each biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test. Figure 5—source data 1.The western blot raw images in Figure 5.Figure 5—source data 2.The labeled western blot images in Figure 5.Figure 5—source data 3.Excel file containing summary data and data analysis of Figure 5.The western blot raw images in Figure 5.The labeled western blot images in Figure 5.Excel file containing summary data and data analysis of Figure 5. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF) induced the claudin-5 expression in hCMEC/D3 cells by activating the PI3K/AKT/FOXO1 pathway. Effects of US-CM and GDNF on the cytoplasmic p-FOXO1, cytoplasmic FOXO1, and nuclear FOXO1 expression (B). Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of GDNF by Western Blot

Detection of GDNF by Western Blot

Glial cell line-derived neurotrophic factor (GDNF)-induced VE-cadherin expression in hCMEC/D3 cells by activating the PI3K/AKT/ETS1 and MAPK/ERK/ETS1 pathways. Effects of US-CM and GDNF on total (A) and nuclear (B) ETS1 expression. Effects of LY and U0 on 200 pg/ml GDNF-induced total (C) and nuclear (D) ETS1 expression. Expression levels of total (E) and the nuclear ETS1 (F) in hCMEC/D3 cells after knocking down ETS1 with siRNA (siETS1). (G) Effects of GDNF and siETS1 on the expression of VE-cadherin and claudin-5. The above data are shown as the mean ± SEM. Four biological replicates per group. One technical replicate for each biological replicate. *p < 0.05; **p < 0.01 by one-way ANOVA test followed by Fisher’s LSD test. Figure 5—source data 1.The western blot raw images in Figure 5.Figure 5—source data 2.The labeled western blot images in Figure 5.Figure 5—source data 3.Excel file containing summary data and data analysis of Figure 5.The western blot raw images in Figure 5.The labeled western blot images in Figure 5.Excel file containing summary data and data analysis of Figure 5. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/96161), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Rat GDNF Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat dorsal root ganglia

Western Blot

0.1 µg/mL
Sample: Recombinant Human GDNF (Catalog # 212-GD)

Neutralization

Measured by its ability to neutralize GDNF-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND50) is typically 0.25-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.

Reviewed Applications

Read 5 reviews rated 4.6 using AF-212-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Long Name

Glial Cell line-derived Growth Factor

Alternate Names

ATF, HFB1-GDNF, HGDNF, HSCR3

Entrez Gene IDs

2668 (Human); 25453 (Rat)

Gene Symbol

GDNF

UniProt

P39905 (Goat)

Additional GDNF Products

Product Documents for Human/Rat GDNF Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Rat GDNF Antibody

For research use only

Related Research Areas

GDNF Family

Citations for Human/Rat GDNF Antibody

Customer Reviews for Human/Rat GDNF Antibody (5)

4.6 out of 5
5 Customer Ratings
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Showing  1 - 5 of 5 reviews Showing All
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  • Human/Rat GDNF Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Guinea Pig
    Verified Customer | Posted 08/29/2017
    anti h/rGDNF was used to make positive control for competitive elisa to determine GDNF in Guinea pig serum. Works perfect.
    Human/Rat GDNF Antibody AF-212-NA
  • Human/Rat GDNF Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Porcine Serum
    Species: Rat
    Verified Customer | Posted 07/19/2017
    Used for competitive elisa using serum
    Human/Rat GDNF Antibody AF-212-NA
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: Human GDNF bound to the surface of HEK293 cells transfected with the GDNF receptor GFRa1
    Species: Human
    Verified Customer | Posted 12/18/2014
    Immunofluorescent analysis of GDNF in human GDNF (green) bound to the surface of HEK293 cells transfected with the GDNF receptor
    Human/Rat GDNF Antibody AF-212-NA
  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: N/A
    Species: Human
    Verified Customer | Posted 12/18/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Recombinant GDNF binding to 293 cells expressing GDNF receptors or primary hippocampal neurons or brain homogenates
    Species: Human
    Verified Customer | Posted 12/18/2014

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