Human/Rat GDNF Antibody

(25 citations)
(2 Reviews)
  
  • Species Reactivity
    Human, Rat
  • Specificity
    Detects human and rat GDNF in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived and Mouse myeloma cell line NS0-derived recombinant human GDNF
    Arg109-Ile211
    Accession # P39905
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human GDNF (Catalog # 212-GD)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize GDNF-induced proliferation in the SH‑SY5Y human neuroblastoma cell line. The Neutralization Dose (ND50) is typically 0.25-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
GDNF and GFR alpha ‑1 in Rat Dorsal Root Ganglia. GDNF and GFR alpha ‑1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Biotinylated Goat Anti-Rat GFR alpha ‑1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Proliferation Induced by GDNF and Neutralization by Goat GDNF Antibody. Recombinant Human GDNF (Catalog # 212-GD) induces proliferation in the SH‑SY5Y human neuroblastoma cell line in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714-GR) in a dose-dependent manner (orange line), as measured by Rezazurin (Catalog # AR002). Under these conditions, proliferation elicited by GDNF is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-212-NA). The ND50 is typically 0.25-1 μg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

  • Long Name:
    Glial Cell line-derived Growth Factor
  • Entrez Gene IDs:
    2668 (Human); 25453 (Rat)
  • Alternate Names:
    Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; hGDNF; HSCR3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane
    Authors: Y Dong, A Kanda, K Noda, W Saito, S Ishida
    Sci Rep, 2017;7(0):44266.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. Systemic injection of AAV9-GDNF provides modest functional improvements in the SOD1(G93A) ALS rat but has adverse side effects
    Authors: GM Thomsen, M Alkaslasi, JP Vit, G Lawless, M Godoy, G Gowing, O Shelest, CN Svendsen
    Gene Ther, 2017;0(0):.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  3. CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's disease
    Authors: SR Stott, S Hayat, T Carnwath, S Garas, JP Sleeman, RA Barker
    PLoS ONE, 2017;12(2):e0171748.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. beta-Catenin overexpression in the metanephric mesenchyme leads to renal dysplasia genesis via cell-autonomous and non-cell-autonomous mechanisms.
    Authors: Sarin S, Boivin F, Li A, Lim J, Svajger B, Rosenblum N, Bridgewater D
    Am J Pathol, 2014;184(5):1395-410.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair.
    Authors: Wang T, Bruggeman K, Sheean R, Turner B, Nisbet D, Parish C
    J Biol Chem, 2014;289(21):15044-51.
    Species: Human
    Sample Type: Recombinant Protein
    Application: ELISA detection
  6. Clearance and toxicity of recombinant methionyl human glial cell line-derived neurotrophic factor (r-metHu GDNF) following acute convection-enhanced delivery into the striatum.
    Authors: Taylor H, Barua N, Bienemann A, Wyatt M, Castrique E, Foster R, Luz M, Fibiger C, Mohr E, Gill S
    PLoS ONE, 2013;8(3):e56186.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  7. Functional neuroprotection and efficient regulation of GDNF using destabilizing domains in a rodent model of Parkinson's disease.
    Authors: Quintino L, Manfre G, Wettergren E, Namislo A, Isaksson C, Lundberg C
    Mol Ther, 2013;21(12):2169-80.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012;7(6):e39036.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. Interventional MRI-guided putaminal delivery of AAV2-GDNF for a planned clinical trial in Parkinson's disease.
    Authors: Richardson RM, Kells AP, Rosenbluth KH, Salegio EA, Fiandaca MS, Larson PS, Starr PA, Martin AJ, Lonser RR, Federoff HJ, Forsayeth JR, Bankiewicz KS
    Mol. Ther., 2011;19(6):1048-57.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain.
    Authors: Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS
    Neuropharmacology, 2010;58(7):1114-21.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  11. Efficient gene therapy-based method for the delivery of therapeutics to primate cortex.
    Authors: Kells AP, Hadaczek P, Yin D, Bringas J, Varenika V, Forsayeth J, Bankiewicz KS
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(7):2407-11.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Tissue
    Application: IHC
  12. Response of aged parkinsonian monkeys to in vivo gene transfer of GDNF.
    Authors: Emborg ME, Moirano J, Raschke J, Bondarenko V, Zufferey R, Peng S, Ebert AD, Joers V, Roitberg B, Holden JE, Koprich J, Lipton J, Kordower JH, Aebischer P
    Neurobiol. Dis., 2009;36(2):303-11.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Tissue
    Application: IHC Frozen
  13. Direct muscle delivery of GDNF with human mesenchymal stem cells improves motor neuron survival and function in a rat model of familial ALS.
    Authors: Suzuki M, McHugh J, Tork C, Shelley B, Hayes A, Bellantuono I, Aebischer P, Svendsen CN
    Mol. Ther., 2008;16(12):2002-10.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  14. SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
    Authors: Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP
    Development, 2007;134(18):3327-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  15. Identification of neuregulin as a factor required for formation of aligned spermatogonia.
    Authors: Hamra FK, Chapman KM, Nguyen D, Garbers DL
    J. Biol. Chem., 2007;282(1):721-30.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  16. Human neural stem cell grafts ameliorate motor neuron disease in SOD-1 transgenic rats.
    Authors: Xu L, Yan J, Chen D, Welsh AM, Hazel T, Johe K, Hatfield G, Koliatsos VE
    Transplantation, 2006;82(7):865-75.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  17. Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats.
    Authors: Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O
    Stroke, 2006;37(9):2361-7.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  18. Glial cell-line-derived neurotropic factor and its receptors are expressed by germinal and somatic cells of the rat testis.
    Authors: Fouchecourt S, Godet M, Sabido O, Durand P
    J. Endocrinol., 2006;190(1):59-71.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  19. Anatomical basis of glial cell line-derived neurotrophic factor expression in the striatum and related basal ganglia during postnatal development of the rat.
    Authors: Oo TF, Ries V, Cho J, Kholodilov N, Burke RE
    J. Comp. Neurol., 2005;484(1):57-67.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  20. Therapeutic immunization protects dopaminergic neurons in a mouse model of Parkinson's disease.
    Authors: Benner EJ, Mosley RL, Destache CJ, Lewis TB, Jackson-Lewis V, Gorantla S, Nemachek C, Green SR, Przedborski S, Gendelman HE
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(25):9435-40.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  21. Rapid axoglial signaling mediated by neuregulin and neurotrophic factors.
    Authors: Esper RM, Loeb JA
    J. Neurosci., 2004;24(27):6218-27.
    Species: Chicken
    Sample Type: Whole Cells
    Application: Neut
  22. Recombinant adeno-associated viral vector (rAAV) delivery of GDNF provides protection against 6-OHDA lesion in the common marmoset monkey (Callithrix jacchus).
    Authors: Eslamboli A, Cummings RM, Ridley RM, Baker HF, Muzyczka N, Burger C, Mandel RJ, Kirik D, Annett LE
    Exp. Neurol., 2003;184(1):536-48.
    Species: Primate - Callitrix jacchus (Common Marmoset)
    Sample Type: Whole Tissue
    Application: IHC
  23. Response of motoneurons to neonatal sciatic nerve axotomy in Bax-knockout mice.
    Authors: Sun W, Oppenheim RW
    Mol. Cell. Neurosci., 2003;24(4):875-86.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  24. Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate.
    Authors: Honma M, Namikawa K, Mansur K, Iwata T, Mori N, Iizuka H, Kiyama H
    J. Neurochem., 2002;82(4):961-75.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  25. Immunohistochemical localization of GDNF in the human hippocampal formation from prenatal life to adulthood.
    Authors: Pina Serra M, Quartu M, Ambu R, Follesa P, Del Fiacco M
    Brain Res., 2002;928(1):138-46.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
Expand to show all 25 Citations
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Average Rating: 5 (Based on 2 reviews)

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We have 1 review tested in 1 species: Guinea Pig.
We have 2 review tested in 1 application: ELISA.

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Images Ratings Applications Species Reviewed By Date Details
ELISA Human/Rat GDNF Antibody AF-212-NA
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Excellent
 ELISA Guinea Pig Anonymous 08/29/2017
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ELISA Human/Rat GDNF Antibody AF-212-NA
ELISA: Human/Rat GDNF Antibody [AF-212-NA]

Summary

ApplicationELISA
Sample TestedSerum
SpeciesGuinea Pig

Other Experimental Details

Other Experimental Detailsanti h/rGDNF was used to make positive control for competitive elisa to determine GDNF in Guinea pig serum. Works perfect.
ELISA Human/Rat GDNF Antibody AF-212-NA
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Excellent
 ELISA Anonymous 07/19/2017
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ELISA Human/Rat GDNF Antibody AF-212-NA
ELISA: Human/Rat GDNF Antibody [AF-212-NA]

Summary

ApplicationELISA
Sample TestedPorcine Serum

Other Experimental Details

Other Experimental DetailsUsed for competitive elisa using serum

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