Rat IL-17F Antibody

Catalog # Availability Size / Price Qty
IL‑17F in Rat Splenocytes.
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Product Details
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Rat IL-17F Antibody Summary

Species Reactivity
Detects rat IL-17F in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 716728
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant rat IL-17F
Accession # NP_001015011
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL-17F antibody in Rat Splenocytes by Immunocytochemistry (ICC). View Larger

IL‑17F in Rat Splenocytes. IL-17F was detected in immersion fixed Th17-differentiated rat splenocytes using Mouse Anti-Rat IL-17F Monoclonal Antibody (Catalog # MAB4437) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

Interleukin-17F (also ML-1) is a 19 kDa member of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine knot that characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 2, 3, 4). Based on mouse, mature rat IL-17F is 133 amino acids (aa) in length (5, 6). Rat IL-17F is a presumably secreted, 38 kDa glycosylated disulfide-linked homodimer. It is also secreted as a 35 kDa disulfide-linked heterodimer with IL-17/17A (7, 8). The heterodimeric form represents about 30% of secreted IL-17F. Initially, IL-17F was also reported as IL-24. Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature rat IL-17F shares 59% and 90% aa sequence identity with mature human and mouse IL-17F, respectively; it also shares 55% aa identity with rat IL-17. Interspecies studies suggest rat IL-17F is produced by activated Th17-type CD4+ T cells, mast cells, basophils and monocytes (1, 3, 9), and is inducible through the interaction of TGF-beta, IL-6 and IL-23 (9, 10, 11). Targets for IL-17F include respiratory epithelium, fibroblasts, macrophages and endothelial cells which produce proinflammatory cytokines such as GM-CSF, IL-6, IFN-gamma, IP-10, MIP-1 alpha and MCP-1 (2, 6, 12). This activity is found for both homodimeric and heterodimeric forms of IL-17F (7).

  1. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  2. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  3. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:801.
  4. Gaffen, S.H. et al. (2006) Vitam. Horm. 74:255.
  5. Genbank Accession # : NP_001015011.
  6. Oda, N. et al. (2005) Am. J. Respir. Cell Mol. Biol. 171:12.
  7. Chang, S.H. and C. Gong (2007) Cell Res. 17:435.
  8. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  9. Kawaguchi, M. et al. (2001) J. Immunol. 167:4430.
  10. Langrish, C.L. et al. (2005) J. Exp. Med. 201:233.
  11. Yang, X.O. et al. (2007) J. Biol. Chem. 282:9358.
  12. Mcallister, F. et al. (2005) J. Immunol. 175:404.
Long Name
Interleukin 17F
Entrez Gene IDs
112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)
Alternate Names
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F

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