Rat IL-17F Antibody

Catalog # Availability Size / Price Qty
MAB4437
MAB4437-SP
IL‑17F in Rat Splenocytes.
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Rat IL-17F Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IL-17F in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 716728
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat IL-17F
Ala19-Ala153
Accession # NP_001015011
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry IL‑17F in Rat Splenocytes. View Larger

IL‑17F in Rat Splenocytes. IL‑17F was detected in immersion fixed Th17-differentiated rat splenocytes using Mouse Anti-Rat IL‑17F Monoclonal Antibody (Catalog # MAB4437) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

Interleukin-17F (also ML-1) is a 19 kDa member of the IL-17 family of cytokines. Members of this family are involved in tissue homeostasis and demonstrate a structural motif termed a cysteine knot that characterizes a large superfamily of growth factors. Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 2, 3, 4). Based on mouse, mature rat IL-17F is 133 amino acids (aa) in length (5, 6). Rat IL-17F is a presumably secreted, 38 kDa glycosylated disulfide-linked homodimer. It is also secreted as a 35 kDa disulfide-linked heterodimer with IL-17/17A (7, 8). The heterodimeric form represents about 30% of secreted IL-17F. Initially, IL-17F was also reported as IL-24. Since that time, the IL-24 designation has been reassigned to MDA-7, a member of the IL-10 family of molecules (note: IL-17E is synonymous with IL-25). Mature rat IL-17F shares 59% and 90% aa sequence identity with mature human and mouse IL-17F, respectively; it also shares 55% aa identity with rat IL-17. Interspecies studies suggest rat IL-17F is produced by activated Th17-type CD4+ T cells, mast cells, basophils and monocytes (1, 3, 9), and is inducible through the interaction of TGF-beta, IL-6 and IL-23 (9, 10, 11). Targets for IL-17F include respiratory epithelium, fibroblasts, macrophages and endothelial cells which produce proinflammatory cytokines such as GM-CSF, IL-6, IFN-gamma, IP-10, MIP-1 alpha and MCP-1 (2, 6, 12). This activity is found for both homodimeric and heterodimeric forms of IL-17F (7).

References
  1. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  2. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  3. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:801.
  4. Gaffen, S.H. et al. (2006) Vitam. Horm. 74:255.
  5. Genbank Accession # : NP_001015011.
  6. Oda, N. et al. (2005) Am. J. Respir. Cell Mol. Biol. 171:12.
  7. Chang, S.H. and C. Gong (2007) Cell Res. 17:435.
  8. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  9. Kawaguchi, M. et al. (2001) J. Immunol. 167:4430.
  10. Langrish, C.L. et al. (2005) J. Exp. Med. 201:233.
  11. Yang, X.O. et al. (2007) J. Biol. Chem. 282:9358.
  12. Mcallister, F. et al. (2005) J. Immunol. 175:404.
Long Name
Interleukin 17F
Entrez Gene IDs
112744 (Human); 257630 (Mouse); 301291 (Rat)
Alternate Names
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24

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Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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