Rat Jagged 1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
Product Details
Citations (1)
Supplemental Products

Rat Jagged 1 Biotinylated Antibody Summary

Species Reactivity
Detects rat Jagged 1 in ELISAs and Western blots. In sandwich ELISAs, approximately 14% cross-reactivity with recombinant human (rh) Jagged 1 is observed, and less than 0.3% cross-reactivity with rhJagged 2, recombinant mouse Jadded 2, recombinant rat (rr) Notch, and rrNotch 2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant rat Jagged 1
Ser32-Asp1068 (Gly57-Arg59 del, Asp63Thr, Arg64Leu, and Val-Arg-Pro-Tyr ins before Lys69)
Accession # Q63722
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG)

Rat Jagged 1 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Rat Jagged 1 Antibody (Catalog # AF599)

Standard: Recombinant Rat Jagged 1 Fc Chimera Protein, CF (Catalog # 599-JG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jagged 1

Rat Jagged 1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged 1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region (1). The extracellular region of rJagged 1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (2). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (3). For instance soluble, non-transmembrane forms of Jagged 1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (4). Soluble Jagged 1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (5). Furthermore, Jagged 1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged 1 (6). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (7). Rat Jagged 1 shows 98% and 99% aa identity to human and mouse Jagged 1 extracellular domains respectively. Relative to the extracellular region of rat Jagged 2, the aa identity is 58%.

  1. Lindsell, C.E. et al. (1995) Cell 80:909.
  2. Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
  3. Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
  4. Small, D. et al. (2001) J. Biol. Chem. 276:32022.
  5. Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
  6. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
  7. Hicks, C. et al. (2002) J Neurosci. Res. 68:655.
Entrez Gene IDs
182 (Human); 16449 (Mouse); 29146 (Rat)
Alternate Names
AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1

Product Datasheets

You must select a language.


Citation for Rat Jagged 1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Normalization of Notch upregulation reverts large vessels to microvessels via EphB4-mediated venous reprogramming
    Authors: Patrick A. Murphy, Tyson N. Kim, Gloria Lu, Andrew W. Bollen, Chris B. Schaffer, Rong A. Wang
    Science Translational Medicine


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Rat Jagged 1 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Rat Jagged 1 Biotinylated Antibody and earn rewards!

Have you used Rat Jagged 1 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review