Rat MCAM/CD146 Biotinylated Antibody

    
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  • Species Reactivity
    Rat
  • Specificity
    Detects rat MCAM/CD146 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human MCAM is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
    Arg19-Lys560
    Accession # Q9EPF2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat MCAM/CD146
  • Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry. PC‑12 rat adrenal pheo­chromocytoma cell line was stained with Rat MCAM/CD146 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF3250, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MCAM/CD146

MCAM, also known as CD146 and MUC18, is a type I transmembrane adhesion molecule belonging to the Ig superfamily. It is expressed on all endothelial cells, different epithelial cells, multiple lymphocyte subsets and various tumor cells. Two splice isoforms that differ in their cytoplasmic domains and vasolateral localization have been described. A soluble MCAM is shed and can be detected in circulation. The amino acid sequence of rat MCAM is 72% and 90% identical to that of human and mouse MCAM, respectively.

  • Long Name:
    Melanoma Cell Adhesion Molecule
  • Entrez Gene IDs:
    4162 (Human); 84004 (Mouse); 78967 (Rat)
  • Alternate Names:
    CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin
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FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 4
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NorthernLights Streptavidin NL557

Flow , IHC , ICC NL999 4
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NorthernLights Streptavidin NL493

Flow , IHC , ICC NL997 2
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Streptavidin-Allophycocyanin

Flow F0050 1
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NorthernLights Streptavidin NL637

Flow , IHC , ICC NL998 2
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999 4
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 50  
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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