Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry.
PC‑12 rat adrenal pheochromocytoma cell line was stained with Rat MCAM/CD146 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF3250, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MCAM, also known as CD146 and MUC18, is a type I transmembrane adhesion molecule belonging to the Ig superfamily. It is expressed on all endothelial cells, different epithelial cells, multiple lymphocyte subsets and various tumor cells. Two splice isoforms that differ in their cytoplasmic domains and vasolateral localization have been described. A soluble MCAM is shed and can be detected in circulation. The amino acid sequence of rat MCAM is 72% and 90% identical to that of human and mouse MCAM, respectively.
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