MCAM, also known as CD146 and MUC18, is a type I transmembrane adhesion molecule belonging to the Ig superfamily. It is expressed on all endothelial cells, different epithelial cells, multiple lymphocyte subsets and various tumor cells. Two splice isoforms that differ in their cytoplasmic domains and vasolateral localization have been described. A soluble MCAM is shed and can be detected in circulation. The amino acid sequence of rat MCAM is 72% and 90% identical to that of human and mouse MCAM, respectively.
Key Product Details
Species Reactivity
Rat
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
Arg19-Lys560
Accession # Q9EPF2
Arg19-Lys560
Accession # Q9EPF2
Specificity
Detects rat MCAM/CD146 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human MCAM is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat MCAM/CD146 Antibody
Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry.
PC-12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Rat MCAM/CD146 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3250, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).Applications for Rat MCAM/CD146 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: PC‑12 rat adrenal pheochromocytoma cell line
Sample: PC‑12 rat adrenal pheochromocytoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Rat MCAM/CD146
Sample: Recombinant Rat MCAM/CD146
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: MCAM/CD146
Long Name
Melanoma Cell Adhesion Molecule
Alternate Names
CD146, L-Gicerin, MUC18
Gene Symbol
MCAM
UniProt
Additional MCAM/CD146 Products
Product Documents for Rat MCAM/CD146 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Rat MCAM/CD146 Antibody
For research use only
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Protocols
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Associated Pathways
