Rat Serpin A6 Antibody

Catalog # Availability Size / Price Qty
MAB3838
MAB3838-SP
Detection of Rat Serpin A6 by Western Blot.
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Rat Serpin A6 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Serpin A6 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 930816
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Serpin A6
Met1-Ala396
Accession # P31211
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Rat Serpin A6 by Western Blot. View Larger

Detection of Rat Serpin A6 by Western Blot. Western blot shows lysates of rat olfactory bulb tissue, rat liver tissue, rat brain (cerebellum) tissue, and rat brain (hippocampus) tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin A6 at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Serpin A6 in Rat Brain. View Larger

Serpin A6 in Rat Brain. Serpin A6 was detected in perfusion fixed frozen sections of rat brain (preoptic nucleus) using Mouse Anti-Rat Serpin A6 Monoclonal Antibody (Catalog # MAB3838) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A6

Serpin A6, also known as Corticosteroid-binding Globulin/CBG and Transcortin, is a 45‑60 kDa secreted glycoprotein member of the clade A serpin family of protease inhibitors. Although it is a serpin family member, it shows no protease inhibition. Serpin A6 is produced principally by hepatocytes, but is also expressed by hypothalamic neurons and anterior pituitary endocrine cells. Serpin A6 transports progesterone and > 90% of circulating cortisol, and likely has three functions: first it can be cleaved, releasing cortisol to the tissues; second, it regulates levels of free hormone available over time; and third, it likely binds to specific cell-surface receptors when complexed to cortisol. Mature rat Serpin A6 is 374 amino acids (aa) in length (aa 23‑396). It contains an RCL segment that is involved in steroid binding (aa 334‑351). Over aa 23‑396, rat Serpin A6 shares 78% and 60% aa sequence identity with mouse and human Serpin A6, respectively.

Entrez Gene IDs
866 (Human); 12401 (Mouse); 299270 (Rat)
Alternate Names
antitrypsin), member 6; CBG; CBGcorticosteroid-binding globulin; corticosteroid binding globulin; member 6; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A6; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); transcortin

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

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