Rat TIM-1/KIM-1/HAVCR Antibody

Catalog # Availability Size / Price Qty
MAB3689-SP
MAB3689-100
MAB3689-500
Product Details
Citations (2)
FAQs
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Rat TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat TIM-1/KIM-1/HAVCR in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse (rm) TIM‑1, rmTIM-3, rmTIM-4, recombinant human (rh) TIM‑1, rhTIM-4, or rhNKp44 is observed.
Source
Monoclonal Mouse IgG1 Clone # 511227
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse Myeloma cell line NS0-derived recombinant rat TIM-1/KIM-1/HAVCR
Ser18-Val238
Accession # O54947
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Rat TIM-1/KIM-1/HAVCR Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Rat TIM‑1/KIM‑1/HAVCR Biotinylated Antibody (Catalog # BAF3689)

Standard: Recombinant Rat TIM-1/KIM-1/HAVCR Protein, CF (Catalog # 3689-TM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM-1, also known as KIM-1 (Kidney-injury molecule-1) and HAVCR, is a 50-80 kDa, variably glycosylated, type I transmembrane glycoprotein that is a member of the TIM family of immunoglobulin superfamily molecules (1‑5). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM/KIM genes (# 1‑8) vs. only three genes in human (# 1, 3, 4) (1, 2, 5). It is unknown if the rat genome exactly parallels that of mouse. Rat TIM-1 is synthesized as a 307 amino acid (aa) precursor that contains a 21 aa signal sequence, a 214 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (4). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple Thr-Ser-Pro motifs. The mucin region may undergo extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and this may be reflected in rat (4, 6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing an 85 kDa soluble molecule (7). A similar process has now been described in rat (8). The ECD of rat TIM-1 is 50% and 81% aa identical to human and mouse TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (9, 10). However, others are believed to exist, and based on the ligand for TIM-3, one might be an S-type lectin (11). TIM-1 is found on CD4+ T cells and proximal renal tubular cells (4, 12). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 11). In particular, it induces IL-4 production, and requires the TIM-1 cytoplasmic tyrosine phosphorylation motif (12). Alternatively, TIM-1 activation of TIM-4 induces cell cycle arrest (13).

References
  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
  2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
  3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
  4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
  5. Kuchroo, V.K. et al. (2006) Adv. Immunol. 91:227.
  6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  8. Vaidya, V.S. et al. (2006) Am. J. Physiol. Renal Physiol. 290:F517.
  9. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  10. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  11. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  12. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113.
  13. Mesri, M. et al. (2006) Int. Immunol. 18:473.
Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

Product Datasheets

Citations for Rat TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Bone Marrow-Derived Mononuclear Cell Therapy Accelerates Renal Ischemia-Reperfusion Injury Recovery by Modulating Inflammatory, Antioxidant and Apoptotic Related Molecules
    Authors: FM Ornellas, DS Ornellas, SV Martini, RC Castiglion, GM Ventura, PR Rocco, B Gutfilen, SA de Souza, CM Takiya, MM Morales
    Cell. Physiol. Biochem., 2017;41(5):1736-1752.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Lipopolysaccharide-Induced Acute Kidney Injury Is Dependent on an IL-18 Receptor Signaling Pathway
    Authors: Y Nozaki, S Hino, J Ri, K Sakai, Y Nagare, M Kawanishi, K Niki, M Funauchi, I Matsumura
    Int J Mol Sci, 2017;18(12):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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