RbAp46 Antibody

Novus Biologicals | Catalog # NB120-3535

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Herring

Cited:

Human

Predicted:

Rat (100%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RbAp46 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) A S K E M F E D T V E E(13) C of human RbAp46.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (84%). Mouse reactivity reported in scientific literature (PMID: 14645126).

Localization

Nuclear.

Specificity

NB120-3535 detects retinoblastoma protein (Rb) associated protein 46 (RbAp46) from human cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RbAp46 Antibody

Western Blot: RbAp46 Antibody [NB120-3535]

Western Blot: RbAp46 Antibody [NB120-3535]

Western Blot: RbAp46 Antibody [NB120-3535] - Analysis of RbAp46 on 1) Du-145 2) LNCaP and 3) Hep-G2 cell extracts.
Immunocytochemistry/ Immunofluorescence: RbAp46 Antibody [NB120-3535]

Immunocytochemistry/ Immunofluorescence: RbAp46 Antibody [NB120-3535]

Immunocytochemistry/Immunofluorescence: RbAp46 Antibody [NB120-3535] - Immunolocalization of RbAp46 in HeLa cells.
Western Blot: RbAp46 Antibody [NB120-3535]

Western Blot: RbAp46 Antibody [NB120-3535]

Western Blot: RbAp46 Antibody [NB120-3535] - Figure 1: IF of RbAp46 in HeLa cells. Figure 2: WB of RbAp46 on 1) Du-145 2) LNCaP and 3) Hep-G2 cell extracts.

Applications for RbAp46 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1.0 ug/ml

Simple Western

1:50

Western Blot

0.5 ug/ml
Application Notes

See Simple Western Antibody Database for Simple Western validation: Tested in Blood, separated by Size, antibody dilution of 1:50

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RbAp46

Retinoblastoma protein (Rb) associated protein 46 (RbAp 46) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp 46 shares about 75% sequence identity with the closely related RbAp 48, and appears to be functionally related to the yeast MSI 1 protein in that overexpression of RbAp 46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp 46 has also been demonstrated to be up-regulated by the Wilms' tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp46 have shown strong inhibition of growth.

Alternate Names

G1/S transition control protein-binding protein RbAp46, Histone acetyltransferase type B subunit 2, histone-binding protein RBBP7, MGC138867, Nucleosome-remodeling factor subunit RBAP46, RBAP46, RBBP-7, retinoblastoma binding protein 7, Retinoblastoma-binding protein 7MGC138868, Retinoblastoma-binding protein p46, retinoblastoma-binding protein RbAp46

Gene Symbol

RBBP7

Additional RbAp46 Products

Product Documents for RbAp46 Antibody

Certificate of Analysis

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Product Specific Notices for RbAp46 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RbAp46 Antibody

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Protocols

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