RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-41201

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chromatin Immunoprecipitation (ChIP), Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 13D10

Format

Azide and BSA Free
View all RBBP4 Primary Antibodies »

Product Specifications

Immunogen

The complete coding region (amino acids 1-425) of RBBP4/RbAp48 expressed in E. coli.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34205080).

Localization

Nuclear

Specificity

This antibody will recognize human RbAp48.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free

Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]

Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]

Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
RBBP4/RbAp48 Antibody (13D10)

Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -

Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -SH-SY5Y whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] -

RbAp48 antibody [13D10] detects RbAp48 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RbAp48 protein stained by RbAp48 antibody [13D10] (NBP1-41201) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Applications for RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Reactivity reported in (PMID: 30551478)

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunoprecipitation

1:500

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-41201 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBBP4

RbAp48-13D10 recognizes full-length RbAp48, a 48a nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

Long Name

Retinoblastoma Binding Protein 4

Alternate Names

CAF-1 p48, CAF-1C, NURF55, RBAP48

Entrez Gene IDs

5928 (Human)

Gene Symbol

RBBP4

Additional RBBP4 Products

Product Documents for RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free

Customer Reviews for RBBP4/RbAp48 Antibody (13D10) - Azide and BSA Free (1)

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  • Name: Fugui Li
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 09/23/2014

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