RBBP4/RbAp48 Antibody (15G12) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-41202

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 15G12

Format

Azide and BSA Free
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Product Specifications

Immunogen

The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

This antibody will recognize human RbAp48 + RbAp46.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for RBBP4/RbAp48 Antibody (15G12) - Azide and BSA Free

RBBP4/RbAp48 Antibody (15G12)

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] -

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - RBBP4/RbAp48 antibody [15G12] detects RBBP4/RbAp48 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RBBP4/RbAp48 stained by RBBP4/RbAp48 antibody [15G12] (NBP1-41202) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody diluted at 1:1000.
Scale bar= 10um.
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - RbAp48 + RbAp46 antibody detects RbAp48 + RbAp46 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. RbAp48 + RbAp46 stained by RbAp48 + RbAp46 antibody diluted at 1:100.
Immunohistochemistry: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202]

Immunohistochemistry: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202]

Immunohistochemistry: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - Analysis of paraffin-embedded H1299 xenograft, using RbAp48 antibody at 1:200 dilution.
Immunohistochemistry: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202]

Immunohistochemistry: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202]

Immunohistochemistry: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - Analysis of paraffin-embedded C2C12 xenograft, using RbAp48 antibody at 1:200 dilution.
RBBP4/RbAp48 Antibody (15G12)

Western Blot: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] -

Western Blot: RBBP4/RbAp48 Antibody (15G12) [NBP1-41202] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RBBP4/RbAp48 antibody [15G12] (NBP1-41202) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for RBBP4/RbAp48 Antibody (15G12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

0.2-1 ug/ml

Western Blot

1:500-1:3000
Application Notes
EMSA. WB: Predicted MW is 47.6 kDa.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBBP4

RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.

Long Name

Retinoblastoma Binding Protein 4

Alternate Names

CAF-1 p48, CAF-1C, NURF55, RBAP48

Entrez Gene IDs

5928 (Human)

Gene Symbol

RBBP4

Additional RBBP4 Products

Product Documents for RBBP4/RbAp48 Antibody (15G12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RBBP4/RbAp48 Antibody (15G12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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