RBBP4/RbAp48 Antibody (JU37-47)

Novus Biologicals | Catalog # NBP2-75642

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JU37-47 expressed in HEK293
View all RBBP4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein within Human RBBP4/RbAp48 aa 1-98 / 425. (SwissProt: Q09028 Human; SwissProt: Q60972 Mouse)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RBBP4/RbAp48 Antibody (JU37-47)

Western Blot: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Western Blot: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Western Blot: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution.Positive control:Lane 1: NIH-3T3 Lane 2: HelaLane 3: Rat brain Lane 4: Mouse testis
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
Flow Cytometry: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Flow Cytometry: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Flow Cytometry: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642]

Immunohistochemistry-Paraffin: RBBP4/RbAp48 Antibody (JU37-47) [NBP2-75642] - Analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.

Applications for RBBP4/RbAp48 Antibody (JU37-47)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1:10-1:50

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBBP4

RbAp48 encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

Long Name

Retinoblastoma Binding Protein 4

Alternate Names

CAF-1 p48, CAF-1C, NURF55, RBAP48

Gene Symbol

RBBP4

Additional RBBP4 Products

Product Documents for RBBP4/RbAp48 Antibody (JU37-47)

Certificate of Analysis

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Product Specific Notices for RBBP4/RbAp48 Antibody (JU37-47)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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