Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RbBP5 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 538) of human retinoblastoma binding protein 5 using the numbering given in entry NP_005048.2 (GeneID 5929).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RbBP5 Antibody - BSA Free

Western Blot: RbBP5 Antibody [NB600-252]

Western Blot: RbBP5 Antibody [NB600-252]

Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252]

Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252]

Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Chromatin Immunoprecipitation Sequencing: RbBP5 Antibody [NB600-252] - Localization of RbBP5 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-RbBP5 antibody NB600-252 and analyzed by DNA sequencing. The figure illustrates the peak distribution of RbBP5 binding within a 500 Kb region of chromosome 1 as detected using anti-RbBP5 antibody NB600-252. ChIP-seq validation performed by Diogenode, Denville, NJ.
Western Blot: RbBP5 Antibody [NB600-252]

Western Blot: RbBP5 Antibody [NB600-252]

Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Chromatin Immunoprecipitation: RbBP5 Antibody [NB600-252] - Chromatin Immunoprecipitation: RbBP5 Antibody [NB600-252] - ChIP-chip scatter plot of anti-RbBP5 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-252 was used to immunoprecipitate chromatin from K562 cells per Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-RbBP5 ChIP, normal rabbit IgG showed little enrichment.

Applications for RbBP5 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

10 ug

Chromatin Immunoprecipitation Sequencing

2 ug

Immunocytochemistry/ Immunofluorescence

1-4 ug/ml

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:10000 - 1:25000
Application Notes
Reported use in ChIP assays (see Dou et al). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB600-252 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RbBP5

The protein encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B.

Alternate Names

RBQ3RBBP-5, retinoblastoma binding protein 5, Retinoblastoma-binding protein RBQ-3, Set1c WD40 repeat protein, homolog, SWD1

Entrez Gene IDs

5929 (Human); 213464 (Mouse)

Gene Symbol

RBBP5

UniProt

Q15291 (Human)

Additional RbBP5 Products

Product Documents for RbBP5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RbBP5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RbBP5 Antibody - BSA Free

Customer Reviews for RbBP5 Antibody - BSA Free (1)

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  • Name: Koustav Sarkar
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 03/26/2015

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Protocols

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