RCOR1/CoREST Antibody (9B3R7)

Novus Biologicals | Catalog # NBP3-16225

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9B3R7 expressed in HEK293
View all RCOR1/CoREST Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RCOR1/CoREST (Q9UKL0). MPAMVEKGPEVSGKRRGRNNAAASASAAAASAAASAACASPAATAASGAAASSASAAAASAAAAPNNGQNKSLAAAAPNGNSSSNSWEEGSSGSSSDEEH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RCOR1/CoREST Antibody (9B3R7)

Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225]

Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225]

Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of extracts of C2C12 cells, using RCOR1/CoREST Rabbit mAb (NBP3-16225) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225]

Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225]

Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of extracts of various cell lines, using RCOR1/CoREST Rabbit mAb (NBP3-16225) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225]

Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225]

Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of 300ug extracts of THP-1 cells using 3ug RCOR1/CoREST antibody (NBP3-16225). Western blot was performed from the immunoprecipitate using RCOR1/CoREST antibody (NBP3-16225) at a dilition of 1:1000.
RCOR1/CoREST Antibody (9B3R7)

Chromatin Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] -

Chromatin Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RCOR1/CoREST antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
RCOR1/CoREST Antibody (9B3R7)

Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] -

Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using RCOR1/CoREST Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
RCOR1/CoREST Antibody (9B3R7)

Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] -

Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RCOR1/CoREST Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
RCOR1/CoREST Antibody (9B3R7)

Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] -

Immunohistochemistry: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using RCOR1/CoREST Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for RCOR1/CoREST Antibody (9B3R7)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5ug antibody for 10ug-15ug of Chromatin

ELISA

Recommended starting concentration is 1 μg/mL.

Immunohistochemistry

1:200 - 1:800

Immunohistochemistry-Paraffin

1:200 - 1:800

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RCOR1/CoREST

Brain type II sodium channels are important in neuronal phenotyping and are only seen at high levels in neurons. Studies have shown that CoREST functions as a corepressor for REST. This interaction between REST and CoREST is accomplished by means of a single zinc finger binding motif originating from REST. Together, the REST-CoREST complex mediates long term repression of the type II sodium channel gene (1).

Long Name

REST Corepressor 1

Alternate Names

CoREST, RCOR1

Gene Symbol

RCOR1

Additional RCOR1/CoREST Products

Product Documents for RCOR1/CoREST Antibody (9B3R7)

Certificate of Analysis

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Product Specific Notices for RCOR1/CoREST Antibody (9B3R7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RCOR1/CoREST Antibody (9B3R7)

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Protocols

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FAQs

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