Recombinant Canine IFN-gamma Protein

Formulations:
Catalog # Availability Size / Price Qty
781-CG-050
Product Details
Citations (7)
FAQs
Reviews

Recombinant Canine IFN-gamma Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its anti-viral activity on A‑72 canine fibroma cells infected with vesicular stomatitis virus (VSV). The ED50 for this effect is 0.3-1.5 ng/mL.
Source
E. coli-derived canine IFN-gamma protein
Gln24-Lys166, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
17 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

781-CG

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

781-CG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature canine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 79%‑88% amino acid sequence identity with bovine, equine, and feline IFN-gamma, 62%‑73% with human, porcine, and rhesus IFN-gamma, and 40%-47% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Zucker, K. et al. (1992) J. Interferon Res. 12:191.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Citations for Recombinant Canine IFN-gamma Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Durable Control of Autoimmune Diabetes in Mice Achieved by Intraperitoneal Transplantation of Neo-Islets, Three-Dimensional Aggregates of Allogeneic Islet and Mesenchymal Stem Cells
    Authors: C Westenfeld, A Gooch, Z Hu, J Ahlstrom, P Zhang
    Stem Cells Transl Med, 2017;0(0):.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function
    Authors: KA Lahey, NJ Ronaghan, J Shang, SP Dion, A Désilets, R Leduc, WK MacNaughto
    PLoS ONE, 2017;12(7):e0180259.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Suppression of Canine Dendritic Cell Activation/Maturation and Inflammatory Cytokine Release by Mesenchymal Stem Cells Occurs Via Multiple Distinct Biochemical Pathways
    Stem Cells Dev., 2016;0(0):.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Recombinant Forms of Leishmania amazonensis Excreted/Secreted Promastigote Surface Antigen (PSA) Induce Protective Immune Responses in Dogs
    PLoS Negl Trop Dis, 2016;10(5):e0004614.
    Species: Canine
    Sample Types: Recombinant Protein
    Applications: ELISA (Standard)
  5. Long-lasting protection against canine visceral leishmaniasis using the LiESAp-MDP vaccine in endemic areas of France: double-blind randomised efficacy field trial.
    Authors: Lemesre JL, Holzmuller P, Goncalves RB, Bourdoiseau G, Hugnet C, Cavaleyra M, Papierok G
    Vaccine, 2007;25(21):4223-34.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  6. Protection against experimental visceral leishmaniasis infection in dogs immunized with purified excreted secreted antigens of Leishmania infantum promastigotes.
    Authors: Lemesre JL, Holzmuller P, Cavaleyra M, Goncalves RB, Hottin G, Papierok G
    Vaccine, 2005;23(22):2825-40.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  7. Vaccination with irradiated Ancylostoma caninum third stage larvae induces a Th2 protective response in dogs.
    Authors: Fujiwara RT, Loukas A, Mendez S, Williamson AL, Bueno LL, Wang Y, Samuel A, Zhan B, Bottazzi ME, Hotez PJ, Bethony JM
    Vaccine, 2005;24(4):501-9.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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