Recombinant Canine IL-2 (Cys147Ser) Protein

Formulations:
Catalog # Availability Size / Price Qty
1815-CL-020
Product Details
Citations (1)
FAQs
Reviews (1)

Recombinant Canine IL-2 (Cys147Ser) Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.15‑0.8 ng/mL.
Source
E. coli-derived canine IL-2 protein
Ala21-Thr155 (Cys147Ser), with and without an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met & Ala21
Predicted Molecular Mass
15.6 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1815-CL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1815-CL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-2

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens (1). Since then, it has been shown that IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages, and oligodendrocytes (2). The biological activity of IL-2 is mediated by the binding to cell surface receptor complexes composed of three subunits designated as alpha, beta, and gamma subunits (3). IL-2 binds the  alpha subunit with low affinity. The functional high affinity IL-2 receptor is a heterotrimeric complex of the alpha, beta, and gamma subunits. IL-2 binds with intermediate affinity to the complex containing the  beta and  gamma subunits, which is also capable of transducing IL-2 signals (4). In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex (5). The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, and IL-9 (6). At the amino acid sequence level, canine IL-2 shares 90%, 86%, 85%, 76%, and 75% sequence similarities to feline, human, equine, mouse, and bovine IL-2, respectively (7).

References
  1. Morgan, D.A. et al. (1976) Science 193:1007.
  2. Smith, K.A. et al. (1988) Science 240:1169.
  3. Taniguchi, T. and Y. Minami (1993) Cell 73:5.
  4. Giri, J. et al. (1994) EMBO J. 13:2822.
  5. Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
  6. Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.
  7. Dunham, S.P. et al. (1995) DNA Seq. 5:177.
Long Name
Interleukin 2
Entrez Gene IDs
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF

Citation for Recombinant Canine IL-2 (Cys147Ser) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Safety of AAV factor IX peripheral transvenular gene delivery to muscle in hemophilia B dogs.
    Authors: Haurigot V, Mingozzi F, Buchlis G
    Mol. Ther., 2010;18(7):1318-29.
    Species: Canine
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Canine IL-2 (Cys147Ser) Protein
By Anonymous on 09/07/2018
Application: CellProlif